Algal lipids are considered to be good raw materials towards aqua feed formulation and biofuels production, and hence, they have been investigated for decades. Using external carbon source, microalgal cultivation has been getting attention due to high biomass productivity and lipid content with flexible cultivation of algae. In this present study, a marine microalga, Tetraselmis suecica was cultivated by combined method with sodium acetate as carbon source and reciprocal nutrients concentration. The reciprocal concentration of nitrogen and phosphorus with sodium acetate supply towards lipid and fatty acid methyl esters (FAME) synthesis in terms of live feed efficiency was attempted. The highest FAME content (78.44 ± 1.18% of DW) of alga was obtained in nil nitrogen condition with limited phosphate concentration (N0-PL) followed by N0-P (77.31 ± 2.5% of DW) combination. Limitation of phosphate with combination of nil nitrogen (N0-PL) showed slight increment of total FAME accumulation and its higher increments resulted in mono-unsaturated fatty acids (MUFA) accumulation, indicating nitrogen starvation was a real FAME trigger under heterotrophic cultivation. Effect of heterotrophic cultivated T. suecica feeding on marine copepod Pseudodiaptomus annandalei produced higher population density (119 ± 15 ind. L⁻¹) in N0-P combination and high rate (71.98%) of nauplii production was obtained in N0-PL combination. These findings suggested that the heterotrophic microalgae cultivation can be practiced for aqua feed (especially for copepod cultivations) formulation as well as to phosphorous removal from wastewaters.

中文翻译：

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用含乙酸钠的 N/P 饥饿培养基喂养的砒霜拟螟（Pseudodiaptomus annandalei）的生长增强

藻类脂质被认为是水产饲料配方和生物燃料生产的良好原材料，因此，对它们进行了数十年的研究。使用外部碳源的微藻培养由于藻类的灵活培养和高生物量生产率和脂质含量而受到关注。在本研究中，一种海洋微藻Tetraselmis suecica以醋酸钠为碳源，营养浓度倒数的组合法栽培。就活饲料效率而言，尝试将氮和磷的倒数浓度与乙酸钠供应用于脂质和脂肪酸甲酯 (FAME) 合成。藻类的最高 FAME 含量（78.44 ± 1.18% DW）是在零氮条件下获得的，磷酸盐浓度有限（N0-PL），其次是 N0-P（77.31 ± 2.5% DW）组合。磷酸盐与零氮 (N0-PL) 组合的限制显示总 FAME 积累略有增加，其较高的增加导致单不饱和脂肪酸 (MUFA) 积累，表明氮饥饿是异养培养下真正的 FAME 触发因素。异养栽培的T. suecica的影响以海洋桡足类Pseudodiaptomus annandalei 为食在 N0-P 组合中产生了更高的种群密度 (119 ± 15 ind. L ^-1 )，在 N0-PL 组合中获得了高的无节幼体生产率 (71.98%)。这些发现表明，异养微藻培养可用于水产饲料（尤其是桡足类养殖）配方以及从废水中去除磷。