The emergence of drug-resistant pathogens has urged researchers to discover alternatives for traditional antibiotics. β-amyrin, which is included in the category of triterpenoids extracted from plants, is known for its antimicrobial activity, although the underlying mechanism has not yet been revealed. This study was conducted to elucidate the antifungal mode of action of β-amyrin against Candida albicans. Based on the relevance between triterpenoids and oxidative molecules, reactive oxygen species (ROS) concentrations were detected, which showed a noticeable increment. Disruption of Ca²⁺ homeostasis in the cytosol was additionally analyzed, which was supported by interactions between two. Subsequently, decrease in mitochondrial membrane potential, increment of mitochondrial Ca²⁺, and ROS concentration were monitored, which suggested mitochondrial dysfunction modulated by Ca²⁺. Further investigation confirmed oxidative damage through glutathione reduction and DNA fragmentation. Accumulation of lethal damages resulted in the activation of caspases and externalization of phosphatidylserine, indicating the induction of yeast apoptosis by β-amyrin in C. albicans.

耐药病原体的出现促使研究人员寻找传统抗生素的替代品。β-香树脂醇属于从植物中提取的三萜类化合物，以其抗菌活性而闻名，但其潜在机制尚未揭示。本研究旨在阐明 β-香树脂素对白色念珠菌的抗真菌作用模式。基于三萜类化合物和氧化分子之间的相关性，检测到活性氧 (ROS) 浓度，显示出明显的增加。此外还分析了细胞质中 Ca ²⁺稳态的破坏，这得到了两者之间相互作用的支持。随后，线粒体膜电位降低，线粒体钙离子增加²⁺和 ROS 浓度被监测，这表明由 Ca ²⁺调节的线粒体功能障碍。进一步的研究证实了通过谷胱甘肽还原和 DNA 断裂造成的氧化损伤。致死损伤的积累导致半胱天冬酶的激活和磷脂酰丝氨酸的外化，表明C 中的β-香树脂素诱导酵母细胞凋亡。白种人。