Organochlorine pesticides (OCPs) contaminated sites pose great threats to both human health and environmental safety. Targeted bioremediation in these regions largely depends on microbial diversity and activity. This study applied metagenomics to characterize the microbial communities and functional groups composition features during independent or simultaneous rapeseed oil and tartaric acid applications, as well as the degradation kinetics of OCPs. Results showed that: the degradation rates of α-chlordane, β-chlordane and mirex were better when (0.50% w/w) rapeseed oil and (0.05 mol L⁻¹) tartaric acid were applied simultaneously than singular use, yielding removal rates of 56.4%, 53.9%, and 49.4%, respectively. Meanwhile, bio-stimulation facilitated microbial enzyme (catalase/superoxide dismutase/peroxidase) activity in soils significantly, promoting the growth of dominant bacterial communities. Classification at phylum level showed that the relative abundance of Proteobacteria was significantly increased (p < 0.05). Network analysis showed that bio-stimulation substantially increased the dominant bacterial community's proportion, especially Proteobacteria. The functional gene results illustrated that bio-stimulation facilitated total relative abundance of degradation genes, phosphorus, carbon, nitrogen, sulfur metabolic genes, and iron transporting genes (p < 0.05). In metabolic pathways, functional genes related to methanogenesis and ammonia generation were markedly upregulated, indicating that bio-stimulation promoted the transformation of metabolic genes, such as carbon and nitrogen. This research is conducive to exploring the microbiological response mechanisms of bio-stimulation in indigenous flora, which may provide technical support for assessing the microbial ecological remediation outcomes of bio-stimulation in OCP contaminated sites.

受有机氯农药（OCPs）污染的场所对人类健康和环境安全均构成巨大威胁。这些区域的目标生物修复很大程度上取决于微生物的多样性和活性。这项研究应用了宏基因组学来表征在独立或同时施用菜籽油和酒石酸的过程中微生物群落和官能团的组成特征，以及OCP的降解动力学。结果表明：当菜籽油为（0.50％w / w）和（0.05 mol L ^-1时），α-氯丹，β-氯丹和灭蚁灵的降解率更好。）酒石酸与单独使用同时施用，去除率分别为56.4％，53.9％和49.4％。同时，生物刺激显着促进了土壤中微生物酶（过氧化氢酶/超氧化物歧化酶/过氧化物酶）的活性，促进了优势细菌群落的生长。门类分类表明，变形杆菌的相对丰度显着增加（p <0.05）。网络分析表明，生物刺激显着增加了优势细菌群落的比例，尤其是变形杆菌。功能基因的结果表明，生物刺激促进了降解基因，磷，碳，氮，硫代谢基因和铁转运基因的总相对丰度（p <0.05）。在代谢途径中，与甲烷生成和氨生成有关的功能基因显着上调，表明生物刺激促进了代谢基因如碳和氮的转化。这项研究有助于探索本地菌群中生物刺激的微生物反应机制，这可能为评估OCP污染场所中生物刺激的微生物生态修复结果提供技术支持。