Candida tropicalis is a well–known yeast strain for degradation of polycyclic aromatic hydrocarbons. In this paper, we have reported intensification of biodegradation of pyrene with sonication. This study has also attempted to deduce the mechanistic features of intensification induced by sonication. The initial statistical optimization of biodegradation parameters revealed following results: pH = 6, temperature = 33ºC, agitation speed = 164 rpm and % inoculum = 8.07% v/v and pyrene degradation of ~53%. Investigations of the yeast growth pattern on a wide range of salt concentrations revealed that the strain could withstand upto 20% NaCl concentration. The kinetics of pyrene removal followed the 1^st order kinetics with rate constant of 0.0667 day⁻¹ and half–life of 10.39 days. Optimum sonication duty cycle using flow cytometry was 10% with negligible cell death. Exposure of reaction mixture to 33 kHz sonication in log phase resulted in 30% augmentation in pyrene elimination. The expression of overall proteins present in the samples were examined by SDS–PAGE. This analysis revealed overexpression of catechol 2,3–dioxygenase (–meta route metabolism) in the ultrasound–treated samples. FE–SEM micrographs did not show any cell rupture or damage during sonication.

热带假丝酵母是用于降解多环芳烃的著名酵母菌株。在本文中，我们报道了声处理对in的生物降解作用增强。这项研究还试图推论声处理引起的强化的力学特征。初步统计优化的生物降解参数显示以下结果：pH = 6，温度=33ºC，搅拌速度= 164 rpm，接种量％= 8.07％v / v，degradation降解率为〜53％。在各种盐浓度下对酵母生长模式的研究表明，该菌株可以耐受高达20％的NaCl浓度。芘去除的动力学遵循1^日与0.0667天的速率常数级动力学^-1半衰期为10.39天。使用流式细胞仪的最佳超声处理占空比为10％，细胞死亡可忽略不计。在对数相中将反应混合物暴露于33 kHz超声处理可导致pyr消除增加30％。通过SDS-PAGE检测样品中总蛋白质的表达。该分析显示儿茶酚2,3-双加氧酶的过表达- （元的超声处理的样品中的路线代谢）。FE–SEM显微照片在超声处理过程中未显示任何细胞破裂或损坏。