To evaluate the physiological state of the wild fish inhabiting the Biobío River in South-Central Chile, susceptible to the chemical contamination from different sources, biochemical and physiological biomarkers were applied to wild fish Percilia irwini and Trichomycterus areolatus in situ. Fish caught in the Biobío river in low, medium, and high anthropic impacts areas, with different pollution degrees along the river. Ethoxyresorufin O–O deethylase (EROD) activity was evaluated in fish liver. Length, weight, Gonad weight and Liver weight, Physiological Index, and gill and liver histopathology were conducted. Physicochemical parameters (pH, Temperature, Conductivity, and TDS) were measured at each sampling site. The results indicated a deteriorating condition in the biological parameters of both species in a high anthropic zone. Fishes show an increase in physiological indices and EROD liver activity, agreeing with previous studies supporting evidence of reproductive change development as we move downstream the river. Also, an increase in histopathological lesions towards the lower third stretch of the Biobío River. The Integrated Index of Physiological Biomarkers (IPBR) indicated that sites located in the high impact area (P. irwini: BC: 4.09; RC: 3.38; PC: 3.50; SJ: 2.34 and T. areolatus BC: 6.06, PC: 5.37; SJ: 5.42) have the most detrimental environmental quality, compared to reference area. The integrated biomarker analysis demonstrates that the alterations observed are related to the high anthropic activity levels downstream from the sites with the least intervention, demonstrating that the IPBR used is a complementary tool for studies of the Environmental Effects Monitoring approach.

为了评估居住在智利中南部比奥比奥河（BiobíoRiver）的野鱼的生理状态，该生物易受不同来源的化学污染的影响，将生化和生理生物标记物应用于野鱼Percilia irwini和Trichomycterus areolatus原地。低，中和高人类影响地区的比比奥河中捕获的鱼类，沿河的污染程度不同。在鱼肝中评估了乙氧基间苯二酚的O-O脱乙基酶（EROD）活性。进行长度，体重，性腺重量和肝脏重量，生理指标以及g和肝脏组织病理学检查。在每个采样点测量理化参数（pH，温度，电导率和TDS）。结果表明，在高人类活动区，这两个物种的生物学参数都在恶化。鱼类显示出生理指标和EROD肝脏活性增加，这与以前的研究一致，支持了当我们向河下游移动时生殖变化发展的证据。此外，朝向比奥比奥河下游第三段的组织病理学损害也增加了。。irwini：BC：4.09；RC：3.38。PC：3.50；SJ：2.34，乳臭支原体BC：6.06，PC：5.37；SJ：5.42）与参考区域相比，环境质量最不利。综合的生物标志物分析表明，所观察到的变化与干预最少的场所下游的人类活动水平高有关，表明所使用的IPBR是研究环境影响监测方法的补充工具。