Interference with quorum sensing (QS) represents an antivirulence strategy with a significant promise for the treatment of bacterial infections and a new approach to restoring antibiotic tolerance. Over the past two decades, a novel series of studies have reported that quorum quenching approaches and the discovery of quorum sensing inhibitors (QSIs) have a strong impact on the discovery of anti-infective drugs against various types of bacteria. The discovery of QSI was demonstrated to be an appropriate strategy to expand the anti-infective therapeutic approaches to complement classical antibiotics and antimicrobial agents. For the discovery of QSIs, diverse approaches exist and develop in-step with the scale of screening as well as specific QS systems. This review highlights the latest findings in strategies and methodologies for QSI screening, involving activity-based screening with bioassays, chemical methods to seek bacterial QS pathways for QSI discovery, virtual screening for QSI screening, and other potential tools for interpreting QS signaling, which are innovative routes for future efforts to discover additional QSIs to combat bacterial infections.

对群体感应 (QS) 的干扰代表了一种抗病毒策略，具有治疗细菌感染的重要前景，也是一种恢复抗生素耐受性的新方法。在过去的二十年中，一系列新的研究报告称，群体猝灭方法和群体感应抑制剂 (QSI) 的发现对发现针对各种细菌的抗感染药物产生了重大影响。QSI 的发现被证明是扩展抗感染治疗方法以补充经典抗生素和抗菌剂的适当策略。对于 QSI 的发现，存在多种方法，并与筛选规模以及特定的 QS 系统同步发展。这篇综述重点介绍了 QSI 筛查策略和方法的最新发现，