Micro-structured molecular semiconductor film-based surface-enhanced Raman scattering (SERS) probes are an important analytical tool for both fundamental and technological research. This study proposed a novel zinc oxide (ZnO)-based, three-dimensional (3D) semiconductor nanoflower (NF) superstructure probe with unique physicochemical properties, including engineered hotspots allowing an arrangement of metallic nanoparticles (NPs), as a means to analyse target molecules. By changing the size, high-density hotspots distributed throughout the nanopetal-like ‘nanorods’ of ZnO supports. When used to analyse crystal violet (CV), there were synergistic effects of silver (Ag), ZnO, and CV molecules in the synthesised ZnONFs@Ag-CV SERS system. The SERS results revealed that the plasmonic surfaces of the self-assembled hotspots on the 3D ZnO superstructures provided effective molecular interactions between the ZnONFs@Ag platform and the Raman probe molecule. These interactions influenced the configuration and detection performance of SERS. Moreover, the performance was closely associated with enhancement of the electromagnetic mechanism and the charge transfer contribution in the platform between the semiconductor, metallic NPs, and the analyte molecules. As a result, the characteristic CV peaks were obvious even at a low concentration of 10⁻¹⁰ M. In a mixture of two probes, the ZnONFs@Ag chip provided an outstanding selectivity in the quantitative and qualitative evaluation of each target molecule at low concentrations. The synthesised 3D ZnONFs@Ag heterostructure chip possesses excellent practical reproducibility and represents a promising candidate for chemical and biomedical inspection.

基于微结构分子半导体薄膜的表面增强拉曼散射 (SERS) 探针是基础研究和技术研究的重要分析工具。本研究提出了一种新型的基于氧化锌 (ZnO) 的三维 (3D) 半导体纳米花 (NF) 超结构探针，该探针具有独特的物理化学特性，包括允许排列金属纳米颗粒 (NP) 的工程热点，作为分析目标的一种手段。分子。通过改变尺寸，高密度热点分布在 ZnO 载体的纳米花瓣状“纳米棒”中。当用于分析结晶紫 (CV) 时，合成的 ZnONFs@Ag-CV SERS 系统中存在银 (Ag)、ZnO 和 CV 分子的协同效应。SERS 结果表明，3D ZnO 超结构上自组装热点的等离子体表面在 ZnONFs@Ag 平台和拉曼探针分子之间提供了有效的分子相互作用。这些相互作用影响了 SERS 的配置和检测性能。此外，性能与电磁机制的增强以及半导体、金属纳米颗粒和分析物分子之间平台中的电荷转移贡献密切相关。结果，即使在 10 的低浓度下，特征 CV 峰也很明显。该性能与电磁机制的增强以及半导体、金属 NP 和分析物分子之间平台中的电荷转移贡献密切相关。结果，即使在 10 的低浓度下，特征 CV 峰也很明显。该性能与电磁机制的增强以及半导体、金属 NP 和分析物分子之间平台中的电荷转移贡献密切相关。结果，即使在 10 的低浓度下，特征 CV 峰也很明显。^-10 M。在两种探针的混合物中，ZnONFs@Ag 芯片在低浓度下对每个目标分子的定量和定性评估中提供了出色的选择性。合成的 3D ZnONFs@Ag 异质结构芯片具有出色的实际重现性，是化学和生物医学检测的有希望的候选者。