Nanomaterials (NMs) taken up from the environment carry a complex ecocorona consisting of dissolved organic matter. An ecocorona is assumed to influence the interactions between NMs and endogenous biomolecules and consequently affects the formation of a biological corona (biocorona) and the biological fate of the NMs. This study shows that biomolecules in fish plasma attach immediately (within <5 min) to the surface of SWCNTs and the evolution of the biocorona is a size dependent phenomenon. Quantitative proteomics data revealed that the nanotube size also influences the plasma protein composition on the surface of SWCNTs. The presence of a pre-attached ecocorona on the surface of SWCNTs eliminated the influence of nanotube size on the formation and evolution of the biocorona. Over time, endogenous biomolecules from the plasma partially replaced the pre-attached ecocorona as measured using a fluorescently labelled ecocorona. The presence of an ecocorona offers a unique surface composition to each nanotube. This suggests that understanding the biological fate of NMs taken up from the environment by organisms to support the environmental risk assessment of NMs is a challenging task because each NM may have a unique surface composition in the body of an organism.

从环境中吸收的纳米材料 (NM) 带有由溶解的有机物组成的复杂生态冠。假设生态电晕会影响 NMs 和内源性生物分子之间的相互作用，从而影响生物电晕 (biocorona) 的形成和 NMs 的生物学命运。该研究表明，鱼血浆中的生物分子会立即（在 <5 分钟内）附着在 SWCNT 表面，并且生物电晕的演变是一种尺寸依赖性现象。定量蛋白质组学数据表明，纳米管的尺寸也会影响 SWCNTs 表面的血浆蛋白组成。单壁碳纳米管表面上预先附着的生态电晕的存在消除了纳米管尺寸对生物电晕形成和演化的影响。随着时间的推移，来自血浆的内源性生物分子部分取代了使用荧光标记的生态冠测量的预先附着的生态冠。生态电晕的存在为每个纳米管提供了独特的表面成分。这表明，了解生物体从环境中吸收的 NM 的生物学命运以支持 NM 的环境风险评估是一项具有挑战性的任务，因为每个 NM 在生物体内可能具有独特的表面成分。