ABSTRACT

Some experimental research indicates that low-frequency pulsed electromagnetic field (PEMF) stimulation may accelerate regeneration in sciatic nerve injury. However, little research has examined the electrophysiological and functional properties of regenerating peripheral nerves under PEMF. The main aim of the present study is to investigate the effects of PEMF on sciatic nerve regeneration in short- and long-term processes with electrophysiologically and functionally after crushing damage. Crush lesions were performed using jewelery forceps for 30 s. After crush injury of the sciatic nerves, 24 female Wistar-Albino rats were divided into 3 groups with 8 rats in each group: SH(Sham), SNI (Sciatic Nerve Injury), SNI+PEMF(Sciatic Nerve Injury+Pulsed Electromagnetic Field). SNI+PEMF group was exposed to PEMF (4 h/day, intensity; 0.3mT, low-frequency; 2 Hz) for 40-days. Electrophysiological records (at the beginning and 1st, 2nd, 4th and 6th weeks post-crush) and functional footprints (at 1st, 2nd, 3rd, 4th, 5th and 6th weeks post crush) were measured from all groups during the experiment. The results were compared to SNI and SNI+PEMF groups, it was found that amplitude and area parameters in the first-week were significantly higher and latency was lower in the SNI+PEMF group than in the SNI group (p < 0,05). However, the effect of PEMF was not significant in the 2nd, 4th, 6th weeks. In addition, in the 1st and 2nd weeks, the SSI parameters were significantly higher in SNI+PMF group than SNI group (p < .05). These results indicate that low-frequency PEMF is not effective for long-periods of application time while PEMF may be useful during the short-term recovery period.

摘要

一些实验研究表明，低频脉冲电磁场​​（PEMF）刺激可以加速坐骨神经损伤的再生。然而，很少有研究检查 PEMF 下再生周围神经的电生理和功能特性。本研究的主要目的是研究脉冲电磁场​​对挤压损伤后电生理和功能的短期和长期过程中坐骨神经再生的影响。使用珠宝钳进行挤压损伤 30 秒。坐骨神经挤压伤后，24只雌性Wistar-Albino大鼠分为3组，每组8只：SH(Sham)、SNI(坐骨神经损伤)、SNI+PEMF(坐骨神经损伤+脉冲电磁场​​) . SNI+PEMF组暴露于PEMF（4小时/天，强度；0.3mT，低频；2 Hz) 40 天。在实验期间测量了所有组的电生理记录（在开始和挤压后第 1、2、4 和 6 周）和功能足迹（在挤压后的第 1、2、3、4、5 和 6 周）。结果与SNI和SNI+PEMF组比较，发现SNI+PEMF组在第一周的振幅和面积参数明显高于SNI组，潜伏期更低。p < 0,05）。但PEMF在第2、4、6周效果不显着。此外，在第 1 周和第 2 周，SNI+PMF 组的 SSI 参数显着高于 SNI 组（p < .05）。这些结果表明低频 PEMF 对长期应用时间无效，而 PEMF 在短期恢复期间可能有用。