Lightweight and mechanically strong natural silk fibers have been extensively investigated over the past decades. Inspired by this research, many artificial spinning techniques (wet spinning, dry spinning, electrospinning, etc.) have been developed to fabricate robust protein fibers. As the traditional spinning methods provide poor control over the as-spun fibers, microfluidics has been integrated with these techniques to allow the fabrication of biological fibers in a well-designed manner, with simplicity and cost efficiency. The mechanical behavior of the developed fibers can be precisely modulated by controlling the type iop and size of microfluidic channel, flow rate, and shear force. This technique has been successfully used to manufacture a broad range of protein fibers, and can accelerate the production and application of protein fibers in various fields. This review outlines recent progress in the design and fabrication of protein-based fibers based on microfluidics. We first briefly discuss the natural spider silk-spinning process and the microfluidics spinning process. Next, the fabrication and mechanical properties of regenerated protein fibers via microfluidics are discussed, followed by a discussion of recombinant protein fibers. Other sourced protein fibers are also reviewed in detail. Finally, a brief outlook on the development of microfluidic technology for producing protein fibers is presented.

在过去的几十年中，人们对轻质且机械强度高的天然丝纤维进行了广泛的研究。受这项研究的启发，人们开发了许多人工纺丝技术（湿纺、干纺、静电纺等）来制造坚固的蛋白质纤维。由于传统的纺丝方法对初纺纤维的控制很差，微流体技术已与这些技术相结合，以设计良好的方式制造生物纤维，既简单又具有成本效益。通过控制微流体通道的类型 iop 和大小、流速和剪切力，可以精确调节所开发纤维的机械行为。该技术已成功用于制造多种蛋白质纤维，并且可以加速蛋白质纤维在各个领域的生产和应用。本综述概述了基于微流体的蛋白质纤维设计和制造的最新进展。我们首先简要讨论天然蜘蛛丝纺丝过程和微流体纺丝过程。接下来，讨论了通过微流控再生蛋白质纤维的制造和机械性能，然后讨论了重组蛋白质纤维。还详细审查了其他来源的蛋白质纤维。最后，对蛋白质纤维微流控技术的发展进行了简要展望。我们首先简要讨论天然蜘蛛丝纺丝过程和微流体纺丝过程。接下来，讨论了通过微流控再生蛋白质纤维的制造和机械性能，然后讨论了重组蛋白质纤维。还详细审查了其他来源的蛋白质纤维。最后，对蛋白质纤维微流控技术的发展进行了简要展望。我们首先简要讨论天然蜘蛛丝纺丝过程和微流体纺丝过程。接下来，讨论了通过微流控再生蛋白质纤维的制造和机械性能，然后讨论了重组蛋白质纤维。还详细审查了其他来源的蛋白质纤维。最后，对蛋白质纤维微流控技术的发展进行了简要展望。