Channel catfish (Ictalurus punctatus) is the primary culture species in the US along with its hybrid made with male blue catfish, I. furcatus. In an effort to improve the nutritional value of channel catfish, the masou salmon Δ5-desaturase like gene (D5D) driven by the common carp beta-actin promoter (βactin) was inserted into channel catfish. The objectives of this study were to determine the effectiveness of βactin-D5D for improving n-3 fatty acid production in F1 transgenic channel catfish, as well as examine pleiotropic effects on growth, proximate analysis, disease resistance, and other performance traits. Transgenic F1 channel catfish showed a 33% increase in the relative proportion of n-3 fatty acids coupled with a 15% decrease in n-6 fatty acids and a 17% decrease in n-9 fatty acids when compared to non-transgenic full-siblings (P < 0.01, P < 0.01, P < 0.01). However, while the relative proportion of n-3 fatty acids was achieved, the total amount of fatty acids in the transgenic fish decreased resulting in a reduction of all fatty acids. Insertion of the βactin-D5D transgene into channel catfish also had large effects on the body composition, and growth of channel catfish. Transgenic channel catfish grew faster, were more disease resistant, had higher protein and moisture percentage, but lower fat percentage than full-sib controls. There were sex effects as performance changes were more dramatic and significant in males. The βactin-D5D transgenic channel catfish were also more uniform in their fatty acid composition, growth and other traits.

沟鲶 ( Ictalurus punctatus ) 是美国的主要养殖品种，它与雄性蓝鲶鱼I. furcatus杂交. 为了提高鲶鱼的营养价值，由鲤鱼 β-肌动蛋白启动子 (βactin) 驱动的马苏鲑鱼 Δ5-去饱和酶样基因 (D5D) 被插入到鲶鱼中。本研究的目的是确定 βactin-D5D 对提高 F1 转基因鲶鱼 n-3 脂肪酸产量的有效性，并检查对生长、近似分析、抗病性和其他性能特征的多效性影响。与非转基因全脂鲶鱼相比，转基因 F1 鲶鱼的 n-3 脂肪酸的相对比例增加了 33%，同时 n-6 脂肪酸的相对比例减少了 15%，n-9 脂肪酸的减少了 17%。兄弟姐妹 ( P  < 0.01, P  < 0.01, P < 0.01)。然而，虽然达到了 n-3 脂肪酸的相对比例，但转基因鱼中脂肪酸的总量下降，导致所有脂肪酸的减少。将 βactin-D5D 转基因插入沟鲶也对沟鲶的身体成分和生长产生很大影响。与全同胞对照相比，转基因鲶鱼生长更快，抗病能力更强，蛋白质和水分百分比更高，但脂肪百分比更低。由于男性的表现变化更加剧烈和显着，因此存在性别影响。βactin-D5D 转基因鲶鱼在脂肪酸组成、生长和其他性状方面也更加一致。