We proposed a method for the extraction of medicinal herbs, called ultrasound-assisted centrifugal extraction, and an online solvent concentration method. These techniques were coupled with two countercurrent chromatography systems and applied to the continuous extraction and online isolation of chemical constituents from Inonotus obliquus. Raw plants were extracted using a two-phase petroleum–ethanol–water (2.0:1.0:2.0, v/v/v) process, and then the aqueous and organic phases were concentrated using the proposed online solvent concentrator. The countercurrent chromatography preparation prior to separation includes pumping of the two-phase solution, rotating column, and equilibrium column. Following online concentration, the extracted solution was pumped into a second countercurrent chromatography process for separation. During separation, the extraction solution and concentrated extract were prepared automatically. Upon completion of the first cycle of ultrasound-assisted centrifugal extraction/two countercurrent chromatography, the second cycle experiment starts. This process can be indefinitely repeated. In this study, six target compounds with purities above 97.71% were successfully extracted and isolated online using a two-phase solvent system consisting of n-hexane-ethyl acetate-acetonitrile (4.5:1.5:5.5, v/v/v) and n-hexane–ethyl acetate–methanol–water (0.4:3.0:1.5:2.5, v/v/v/v). Compared to conventional extraction methods, the instrumental setup of the proposed method provides enhanced automation, efficiency, purity, and systematic extraction and isolation of natural products.

中文翻译：

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开发超声辅助离心萃取结合两种逆流色谱系统同时提取和分离植物化学物质

我们提出了一种提取药材的方法，称为超声辅助离心提取，以及一种在线溶剂浓缩法。这些技术与两个逆流层析系统相结合，并应用于白桦茸化学成分的连续提取和在线分离. 使用石油-乙醇-水两相（2.0:1.0:2.0，v/v/v）工艺提取原始植物，然后使用建议的在线溶剂浓缩器浓缩水相和有机相。分离前的逆流色谱制备包括泵送两相溶液、旋转柱和平衡柱。在线浓缩后，将提取的溶液泵入第二个逆流色谱过程进行分离。在分离过程中，提取液和浓缩提取液是自动制备的。超声辅助离心萃取/双逆流色谱的第一个循环完成后，第二个循环实验开始。这个过程可以无限重复。在本研究中，六种目标化合物的纯度高于 97。Ñ己烷-乙酸乙酯-乙腈（4.5：1.5：5.5，V / V / V）和Ñ己烷-乙酸乙酯-甲醇-水（0.4：3.0：1.5：2.5，V / V / V / V）。与传统的提取方法相比，所提出方法的仪器设置提供了增强的自动化、效率、纯度以及天然产物的系统提取和分离。