Pantoea stewartii subsp. stewartii is the causal agent of the emerging disease in jackfruit crops (Artocarpus heterophyllus L.), known as "Fruit Bronzing". This disease affects the quality of fresh jackfruits, resulting in economic production losses. Thus far, the biology of the P. stewartii subsp. stewartii bacterium during the stages that make up the fruit bronzing disease´s cycle is poorly understood. Therefore, to gain further insight into this emerging disease, we evaluated the association between P. stewartii subsp. stewartii and the jackfruit flea beetle, which could be involved in the dissemination of this bacterium in this crop. Five bacterial isolates were obtained from two of the six groups of field-collected flea beetles (Group I and Group VI). Morphological characteristics and PCR amplification employing taxon-specific primers both indicated the isolates were P. stewartii subsp. stewartii. AG085-I, AG086-I, AG087-I, and AG088-I were isolated from Group I flea beetles, and AG024-VI was isolated from Group VI flea beetles. Molecular analysis results showed that Group I flea beetles correspond to Chaetocnema minuta, while that Group VI flea beetles correspond to the ChrysSA genus. Finally, the molecular identification of bacterial isolates by analysis of the 16S rDNA and cpsD regions showed that the AG085-I, AG086-I, AG087-I, AG088-I isolates correspond to P. ananatis and only the AG024-VI isolate obtained from the Group VI flea beetles ChrysSA corresponds to P. stewartii subsp. stewartii. The AG024-VI isolate showed pathogenic potential against jackfruit. These results demonstrate the presence of CrhysSA sp. flea beetles associated with P. stewartii subsp. stewartii in jackfruit crops.

Pantoea stewartii亚种。stewartii是菠萝蜜作物（Artocarpus heterophyllus L.）中新兴疾病的病因，被称为“水果青铜”。这种疾病影响新鲜菠萝蜜的质量，导致经济生产损失。到目前为止，P。stewartii亚种的生物学。在组成水果烫金病的周期的各个阶段中，对stewartii细菌的了解还很少。因此，为了进一步了解这种新兴疾病，我们评估了P. stewartii亚种之间的关联。斯图瓦蒂菠萝蜜跳蚤甲虫，可能与这种细菌在农作物中的传播有关。从六组田间采集的跳蚤甲虫（第一组和第六组）中获得了五种细菌分离株。形态学特征和使用分类群特异性引物的PCR扩增均表明分离株是斯氏体育杆菌亚种。斯图瓦蒂。从组I的跳蚤甲虫中分离出AG085-1，AG086-I，AG087-I和AG088-I，从组VI的跳蚤甲虫中分离出AG024-VI。分子分析结果表明，第一类跳蚤甲虫对应于小毛象，而第六类跳蚤甲虫对应于ChrysSA。属。最后，通过分析细菌分离株的分子鉴定的16S rDNA和CPSD区域表明，AG085-I，AG086-I，AG087-I，AG088-1分离对应于P.ananatis中并且仅AG024-VI分离物从获得的第六类跳蚤甲虫ChrysSA对应于P. stewartii亚种。斯图尔瓦蒂。AG024-VI分离株显示出对菠萝蜜的致病潜力。这些结果证明了CrhysSA sp。的存在。与P. stewartii亚种相关的跳蚤甲虫。菠萝蜜作物中的stewartii。