MED13L (mediator complex subunit 13-like) is a component of the mediator complex, which functions as a regulator for gene transcription. Since gene abnormalities in MED13L are responsible for neurodevelopmental disorders, MED13L is presumed to play an essential role in brain development. In this study, we prepared a specific antibody against MED13L, anti-MED13L, and analyzed its expression profile in mouse tissues with focusing on the central nervous system. In Western blotting, MED13L exhibited a tissue-dependent expression profile in the adult mouse and was expressed in a developmental stage-dependent manner in brain. In immunofluorescence analyses, MED13L was at least partially colocalized with pre- and post-synaptic markers, synaptophysin, and PSD95, in primary cultured hippocampal neurons. Immunohistochemical analyses revealed that MED13L was relatively highly expressed in ventricular zone surface of cerebral cortex, and was also located both in the cytoplasm and nucleus of neurons in the cortical plate at embryonic day 14. Then, MED13L showed diffuse cytoplasmic distribution throughout the cerebral cortex at the postnatal day (P) 30. In addition, MED13L appeared to be localized in cell type- and developmental stage-specific manners in the hippocampus and cerebellum. These results suggest that MED13L is involved in the development of the central nervous system and synaptic function.
Dev Neurosci

中文翻译：

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介体复合亚基 13 样的表达分析：小鼠大脑发育过程中神经发育障碍的负责基因

MED13L（介体复合物亚基 13 样）是介体复合物的一个组成部分，可作为基因转录的调节剂。由于 MED13L 中的基因异常是导致神经发育障碍的原因，因此推测 MED13L 在大脑发育中起重要作用。在这项研究中，我们制备了一种针对 MED13L 的特异性抗体，即抗 MED13L，并分析了其在小鼠组织中的表达谱，重点是中枢神经系统。在蛋白质印迹中，MED13L 在成年小鼠中表现出组织依赖性表达谱，并在脑中以发育阶段依赖性方式表达。在免疫荧光分析中，MED13L 在原代培养的海马神经元中至少部分与突触前和突触后标志物、突触素和 PSD95 共定位。免疫组化分析显示，MED13L在大脑皮层心室区表面相对高表达，在胚胎第14天也同时位于皮层板神经元的细胞质和细胞核中。然后，MED13L在整个大脑皮层呈弥漫性细胞质分布。出生后一天 (P) 30. 此外，MED13L 似乎以细胞类型和发育阶段特异性方式定位于海马和小脑。这些结果表明 MED13L 参与了中枢神经系统和突触功能的发育。MED13L 在出生后第 30 天 (P) 显示在整个大脑皮层呈弥漫性细胞质分布。此外，MED13L 似乎以细胞类型和发育阶段特异性方式定位于海马和小脑。这些结果表明 MED13L 参与了中枢神经系统和突触功能的发育。MED13L 在出生后第 30 天 (P) 显示在整个大脑皮层呈弥漫性细胞质分布。此外，MED13L 似乎以细胞类型和发育阶段特异性方式定位于海马和小脑。这些结果表明 MED13L 参与了中枢神经系统和突触功能的发育。
开发神经科学