Six extracts were obtained from plant species Hypericum perforatum L., collected at Samsun in Turkey. The aim of this study was to examine the mechanisms of the anticancer activity of these extracts. Methanol, ethyl-acetate and hexane were used as a solvents for extraction from both branch-body part of the plant (extracts 1, 2 and 3) and from plant flowers (extracts 4, 5 and 6). The cytotoxic effects of the extracts were determined against 2D and 3D cancer cell models. Cell cycle changes of treated HeLa cells were analyzed by flow cytometry. Measurements of gene and microRNA expression levels in treated HeLa cells were done by quantitative real time PCR. Five examined extracts (2–6) exerted selective concentration-dependent cytotoxic effects on HeLa, K562, and A549 cancer cells, while the extract 1 exhibited very weak cytotoxicity. The extract 6 showed the highest intensity of cytotoxic activity. All tested extracts (2–6) demonstrated the ability to induce apoptosis in HeLa cells through activation of caspase-3. These extracts remarkably decreased gene expression levels of MMP2, MMP9, TIMP3, and VEGFA in HeLa cells. Flower extracts might have stronger effects on miR128/193a-5p/335 level changes than branch-body extracts. Hypericum perforatum extracts exerted weaker cytotoxic effects on 3D HeLa spheroids when compared with their effects on 2D monolayer HeLa cells. Taken together, results of our research may suggest the promising anticancer properties of the Hypericum perforatum extracts.

从植物物种贯叶连翘获得六种提取物L.，在土耳其的 Samsun 收集。本研究的目的是检查这些提取物的抗癌活性机制。甲醇、乙酸乙酯和己烷用作从植物的枝体部分（提取物 1、2 和 3）和植物花（提取物 4、5 和 6）中提取的溶剂。针对 2D 和 3D 癌细胞模型确定了提取物的细胞毒性作用。通过流式细胞术分析处理过的 HeLa 细胞的细胞周期变化。通过定量实时 PCR 测量处理过的 HeLa 细胞中的基因和 microRNA 表达水平。五种检测的提取物 (2-6) 对 HeLa、K562 和 A549 癌细胞具有选择性的浓度依赖性细胞毒作用，而提取物 1 表现出非常弱的细胞毒性。提取物6显示出最高强度的细胞毒活性。所有测试的提取物 (2-6) 都证明了通过激活 caspase-3 诱导 HeLa 细胞凋亡的能力。这些提取物显着降低了HeLa 细胞中的MMP2、MMP9、TIMP3和VEGFA。花提取物对 miR128/193a-5p/335 水平变化的影响可能比枝体提取物更强。与对 2D 单层 HeLa 细胞的影响相比，贯叶连翘提取物对 3D HeLa 球体的细胞毒性作用较弱。总之，我们的研究结果可能表明贯叶连翘提取物具有良好的抗癌特性。