The recent studies highlighted the critical role of exosomes in the regulation of inflammation. Here, we investigated the dynamic biogenesis of the exosomes in the rat model of asthma. Our finding showed an increase in the expression of IL-4 and the suppression of IL-10 in asthmatic lung tissues compared to the control samples (p < 0.05). Along with the promotion of IL-4, the protein level of TNF-α was induced, showing an active inflammatory status in OVA-sensitized rats. According to our data, the promotion of asthmatic responses increased exosome biogenesis indicated by increased CD63 levels and acetylcholine esterase activity compared to the normal condition (p < 0.05). Data suggest that the stimulation of inflammatory response in asthmatic rats could simultaneously increase the paracrine activity of pulmonary cells via the exosome biogenesis. Exosome biogenesis may correlate with the inflammatory response.

中文翻译：

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哮喘病引起大鼠肺组织外泌体分泌途径的活性

最近的研究强调了外泌体在炎症调节中的关键作用。在这里，我们调查了哮喘大鼠模型中外泌体的动态生物发生。我们的发现表明，与对照样品相比，哮喘肺组织中IL-4的表达增加，IL-10的表达受到抑制（p <0.05）。随着IL-4的促进，TNF-α的蛋白水平被诱导，在OVA致敏的大鼠中表现出活跃的炎症状态。根据我们的数据，与正常情况相比，CD63水平和乙酰胆碱酯酶活性的增加表明，哮喘反应的促进增加了外来体的生物发生（p <0.05）。数据表明，哮喘大鼠中炎症反应的刺激可以通过外来体的生物发生同时增加肺细胞的旁分泌活性。外泌体的生物发生可能与炎症反应有关。