Perfluorobutane sulfonate (PFBS), due to its increasing use as an alternative to perfluooctane sulfonate (PFOS), is widely detected in humans and the environment, necessitating the evaluation of its potential ecotoxicological risk. We assessed the toxicity and bioaccumulation potential of PFBS in Caenorhabditis elegans, using lethality, locomotion, reproduction, life span, growth, and chemotactic behavior as the effect parameters. In addition, a total of 6 generations of exposed parent animals were monitored for locomotion, brood, and life span behaviors. Life span and brood size were significantly reduced in parent nematodes (P0) following exposure to ≥0.1 mM PFBS, but these negative effects did not transfer to the progeny. Although there was no remarkable effect on reproduction and life span in parent worms exposed to ≤0.01 mM PFBS, multigenerational exposure at 0.0005 mM significantly affected the F4 and F5 progeny. Furthermore, 0.01 to 2.0 mM of PFBS substantially retarded the locomotion behavior of P0 worms. At higher concentrations such as 1.0 mM, this negative effect on locomotion was transferred to the next generation (F1) but later recovered from F2 progeny onward. Our findings demonstrate for the first time that chronic exposure to PFBS at higher concentrations can cause behavioral toxicity and could be transferred to the progeny. These findings have significant implications for the environmental risk assessment of PFBS. Environ Toxicol Chem 2021;40:1973–1982. © 2021 SETAC.

中文翻译：

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全氟丁烷磺酸对秀丽隐杆线虫的急性毒性和跨代影响

全氟丁烷磺酸盐 (PFBS) 由于其越来越多地用作全氟辛烷磺酸盐 (PFOS) 的替代品，在人类和环境中被广泛检测到，因此需要对其潜在的生态毒理学风险进行评估。我们评估了 PFBS 在秀丽隐杆线虫中的毒性和生物蓄积潜力，使用杀伤力、运动、繁殖、寿命、生长和趋化行为作为效应参数。此外，共监测了 6 代暴露的亲代动物的运动、育雏和寿命行为。暴露于≥0.1 mM PFBS 后，亲本线虫 (P0) 的寿命和育雏大小显着降低，但这些负面影响并未转移到后代。尽管暴露于 ≤0.01 mM PFBS 对亲代蠕虫的繁殖和寿命没有显着影响，但 0.0005 mM 的多代暴露显着影响 F4 和 F5 后代。此外，0.01 到 2.0 mM 的 PFBS 大大延缓了 P0 蠕虫的运动行为。在更高的浓度（例如 1.0 mM）下，这种对运动的负面影响转移到下一代（F1），但后来从 F2 后代开始恢复。我们的研究结果首次表明，长期暴露于较高浓度的 PFBS 会导致行为毒性，并可能转移给后代。这些发现对 PFBS 的环境风险评估具有重要意义。环境毒物化学2021；40:1973–1982。© 2021 SETAC。