Significance. One of the modern trends in medical diagnostics is based on metabolomics, an approach allowing determination of metabolites which can be the specific features of disease. High-resolution gas spectroscopy allows investigation of the gas metabolite content of samples of biological origin. We present the elaboration of a method of studying diabetic and non-diabetic biological samples, prepared as pellets, by terahertz (THz) high-resolution spectroscopy. Aim: The main idea of the work is studying the content of thermal decomposition gas products of diabetic and non-diabetic dried blood plasma and kidney tissues for revealing the set of gas-markers that characterized the diabetes by the THz high-resolution spectroscopy method. Approach: We present an approach to study the diabetic and non-diabetic blood plasma (human and rats) and kidney tissues (rats), using high-resolution spectroscopy based on the non-stationary effect of THz frequency range. The methods of preparing the blood and kidney tissue samples as pellets and of vaporizing the samples were developed. Results: The measurements of rotational absorption spectra of vapors at heating the pellets prepared from blood and kidney tissue were carried out in 118 to 178 GHz frequency range. The absorption lines appearing in spectra of the sample vapors were detected and identified. The molecular contents of thermal decomposition products differed for non-diabetic and diabetic samples; e.g., main marker is acetone appearing in the diabetic blood (human and rats) and in the diabetic kidney tissue. Conclusions: Our paper illustrates the potential ability for determining the metabolite content of biological samples for diagnostics and prognosis of diseases for clinical medicine.

中文翻译：

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糖尿病和非糖尿病血浆和肾组织颗粒热分解气体产物的太赫兹高分辨率光谱


意义。医学诊断的现代趋势之一是基于代谢组学，这是一种可以确定可能是疾病特定特征的代谢物的方法。高分辨率气体光谱可以研究生物来源样品的气体代谢物含量。我们提出了一种通过太赫兹（THz）高分辨率光谱研究糖尿病和非糖尿病生物样本的方法，这些样本制备为颗粒。目的：该工作的主要思想是研究糖尿病和非糖尿病干血浆和肾组织的热分解气体产物的含量，以通过太赫兹高分辨率光谱方法揭示表征糖尿病的一组气体标记物。方法：我们提出了一种使用基于太赫兹频率范围非平稳效应的高分辨率光谱来研究糖尿病和非糖尿病血浆（人和大鼠）和肾组织（大鼠）的方法。开发了将血液和肾组织样品制备成颗粒以及蒸发样品的方法。结果：在 118 至 178 GHz 频率范围内测量了加热由血液和肾组织制备的颗粒时蒸气的旋转吸收光谱。检测并识别了样品蒸气光谱中出现的吸收线。非糖尿病和糖尿病样品的热分解产物的分子含量不同；例如，主要标志物是出现在糖尿病血液（人和大鼠）和糖尿病肾组织中的丙酮。结论：我们的论文阐明了测定生物样品代谢物含量用于临床医学疾病诊断和预后的潜在能力。