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The role of transcription factor Nrf2 in the toxicity of perfluorooctane sulfonate (PFOS) and perfluorooctanoic acid (PFOA) in C57BL/6 mouse astrocytes
Environmental Toxicology and Pharmacology ( IF 4.2 ) Pub Date : 2021-04-01 , DOI: 10.1016/j.etap.2021.103652
Saif A Alharthy 1 , Diane Hardej 2
Affiliation  

Perfluorooctane sulfonate (PFOS) and perfluorooctanoic acid (PFOA) are members of perfluoroalkyl substances (PFAS). This study aimed to determine the protective role of Nrf2 against the toxicity of these agents. Nrf2-/- and wild-type astrocytes were exposed to PFOS (75−600 μM) and PFOA (400−1000 μM) for 24 h. Lactate dehydrogenase (LDH) release was significantly higher in nrf2-/- than in the wild-type astrocytes. Exposure to 600 μM PFOS and 800 μM PFOA showed significant increases in reactive oxygen species, lipid peroxidation, and apoptosis in nrf2-/- astrocytes as compared to wild-type astrocytes. The GSH/GSSG ratio was significantly decreased in nrf2-/- astrocytes when compared to wild-type astrocytes. Additionally, PFOS and PFOS caused dramatic ultrastructural alterations to the mitochondria. BHT pretreatment in wild-type cells decreased ROS production with exposure to both agents. Results of the present study conclude that PFOS and PFOA are cytotoxic to astrocytes and that nrf2 -/- cells are more sensitive to toxicity by these agents.



中文翻译:


转录因子Nrf2在全氟辛烷磺酸(PFOS)和全氟辛酸(PFOA)对C57BL/6小鼠星形胶质细胞毒性中的作用



全氟辛烷磺酸 (PFOS) 和全氟辛酸 (PFOA) 属于全氟烷基物质 (PFAS)。本研究旨在确定 Nrf2 对这些药物毒性的保护作用。 Nrf2-/-和野生型星形胶质细胞暴露于 PFOS (75−600 μM) 和 PFOA (400−1000 μM) 24 小时。 nrf2 -/- 中的乳酸脱氢酶 (LDH) 释放显着高于野生型星形胶质细胞。与野生型星形胶质细胞相比,暴露于 600 μM PFOS 和 800 μM PFOA 后,nrf2-/-星形胶质细胞中的活性氧、脂质过氧化和细胞凋亡显着增加。与野生型星形胶质细胞相比, nrf2-/-星形胶质细胞的 GSH/GSSG 比率显着降低。此外,全氟辛烷磺酸和全氟辛烷磺酸引起线粒体超微结构的巨大改变。野生型细胞中的 BHT 预处理降低了暴露于这两种试剂的 ROS 产生。本研究的结果得出结论,PFOS 和 PFOA 对星形胶质细胞具有细胞毒性,并且nrf2 -/-细胞对这些药物的毒性更敏感。

更新日期:2021-04-08
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