Abstract

Indoleamine 2,3-dioxygenase1(IDO1) is one of the most important proteins in protect the embryos from the mother's immune system during pregnancy. However, the regulation of the protein expression at the maternal–foetal interface is not fully known. We aimed to study the regulation of IDO1 expression by progesterone in villi and decidua of in early pregnancy. Fifty cases of early pregnancy women's villi and decidua were collected. Tissue explants of chorionic villi and the decidua were cultured in media containing in different concentrations of progesterone, in the presence or absence of mifepristone. Western blot analysis and immunofluorescence were used to detect the expression of IDO1 in chorionic villi and decidua in cultured tissues. IDO1 protein was identified in chorionic villi and decidua tissues of normal pregnant women, and the expression of IDO1in the decidua was significantly higher than those in chorionic villi. Progesterone decreased IDO1 expression in early pregnancy chorionic villi and decidua, and mifepristone, as the progesterone inhibitor, reverted this effect. In normal physiological state of pregnancy, progesterone may be involved in the regulation of immune tolerance by negative regulation of IDO1 expression at maternal foetal interface. Progesterone may down-regulate IDO1 expression during early pregnancy.

摘要

Indoleamine 2,3-dioxygenase1 (IDO1) 是在怀孕期间保护胚胎免受母亲免疫系统影响的最重要的蛋白质之一。然而，母胎界面蛋白质表达的调节尚不完全清楚。我们旨在研究孕激素在妊娠早期绒毛和蜕膜中对 IDO1 表达的调节。收集了50例早孕妇女绒毛和蜕膜。在存在或不存在米非司酮的情况下，将绒毛膜绒毛和蜕膜的组织外植体在含有不同浓度黄体酮的培养基中培养。采用Western印迹分析和免疫荧光法检测培养组织中绒毛膜和蜕膜组织中IDO1的表达。在正常孕妇的绒毛膜绒毛和蜕膜组织中鉴定到 IDO1 蛋白，IDO1在蜕膜中的表达明显高于绒毛膜。孕酮降低了妊娠早期绒毛膜绒毛和蜕膜中 IDO1 的表达，而作为孕酮抑制剂的米非司酮则逆转了这种作用。在正常妊娠生理状态下，孕激素可能通过负调控母胎界面IDO1表达参与免疫耐受的调节。孕激素可能会在妊娠早期下调 IDO1 的表达。孕激素可能通过负调控母胎界面的 IDO1 表达参与免疫耐受的调节。孕激素可能会在妊娠早期下调 IDO1 的表达。孕激素可能通过负调控母胎界面的 IDO1 表达参与免疫耐受的调节。孕激素可能会在妊娠早期下调 IDO1 的表达。