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DArTseq-derived SNPs for the genus Psidium reveal the high diversity of native species
Tree Genetics & Genomes ( IF 1.9 ) Pub Date : 2021-03-31 , DOI: 10.1007/s11295-021-01505-y
Luara Lopes Grossi , Miquéias Fernandes , Matheus Alves Silva , Carolina de Oliveira Bernardes , Amélia Carlos Tuler , Pedro Henrique Dias dos Santos , Adésio Ferreira , Marcia Flores da Silva Ferreira

In this study, we characterized DArTseq-derived SNPs for species of Psidium, an important Neotropical genus within the family Myrtaceae. Nine Psidium species were used for the identification and characterization of an interspecific set of SNPs. The samples chosen represented a wide range of variation regarding agronomic traits and geographical origin. The samples were genotyped using the DarTseq methodology. This approach, associated with annotation analysis, generated a set of conserved, polymorphic, and possibly functional SNPs. Most of the SNPs in coding regions of Psidium were annotated for the gene ontology classes. We observed high interspecific diversity among species and considerable intraspecific genetic diversity in native species (P. cattleyanum, P. myrtoides, P. acidum, and P. oblongatum). Psidium guajava, a cultivated species, shows low diversity and shares genetic similarities with the tetraploid P. guineense. The group of markers selected in this study will contribute to the development of a panel of interspecific SNPs for Psidium. Our investigation will support further studies regarding genetic variability, diversity, phylogeny, cytogenetics, and other relationships among Psidium species, constituting essential information for the creation, conservation, management, and use of genetic resources in breeding programs.



中文翻译:

r属的DArTseq衍生的SNP揭示了本地物种的高度多样性

在这项研究中,我们的特点为品种DArTseq衍生的SNPs番石榴,在桃金娘科中的一个重要新热带属。九种P属物种用于鉴定和表征种间SNP。所选样品代表了农艺性状和地理起源的广泛变化。使用DarTseq方法对样品进行基因分型。此方法与注释分析相关联,生成了一组保守的,多态的以及可能具有功能的SNP。注释了Psidium编码区中的大多数SNP,用于基因本体分类。我们观察到物种之间的种间高度多样性和天然物种中的相当大的种内遗传多样性(P. cattleyanumP. myrtoidesP.止血,和P. oblongatum)。番石榴(Psidium guajava)是一种栽培种,与四倍体吉尼斯疟原虫P. guineense)相比,多样性低并且具有相似的遗传特性。在这项研究中选择的标记组将有助于开发一个专门用于specific的种间SNPs的小组。我们的研究将支持有关锡si种之间的遗传变异性,多样性,系统发育,细胞遗传学和其他关系的进一步研究,构成在繁殖计划中创建,保存,管理和使用遗传资源的重要信息。

更新日期:2021-03-31
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