Morphological characters of Pama croaker Otolithoides pama were established in the present study. The partial sequence of the fish was generated which is an important record of the species available in the Hooghly-Matlah estuarine system of West Bengal, for any further comparisons. The constructed Neighbor-Joining (NJ) tree based on Kimura’s 2 parameter distance model indicated that O. pama showed an identical phylogenetic relationship with 28 sciaenid species. The tree showed high bootstrap support values with identical topology. The phylogenetic tree constructed for O. pama exhibited high similarity with Panna microdon, Otolithoides biauritus and Pama pama. Within the three closely related species, the overall mean distance between the individuals was found to be 10.30 %. The genetic distance value was highest between Panna microdon and Pama pama (15.60 %) and the lowest genetic distance was observed between Otolithoides biauritus and Panna microdon (0.30 %). The study revealed that for the identification of respective fish species based on nucleotide sequences, Cytochrome c oxidase subunit 1 (COI) genes can be used as barcodes.

中文翻译：

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来自印度西孟加拉邦Hooghly-Matlah河口系统的Otolithoides pama（汉密尔顿，1822年）（Performformes：Sciaenidae）的形态学和分子分析

在本研究中建立了Pama croaker Otolithoides pama的形态学特征。鱼的部分序列已生成，这是西孟加拉邦Hooghly-Matlah河口系统中可用物种的重要记录，以备进一步比较。基于木村的2参数距离模型构建的NJ（Neighbor-Joining，NJ）树表明，O。pama与28种坐骨神经元物种具有相同的系统发生关系。该树显示具有相同拓扑的高引导程序支持值。为O. pama构建的系统发育树与Panna microdon，Otolithoides biauritus和Pama pama表现出高度相似性。在这三个密切相关的物种中，个体之间的总体平均距离为10.30％。在Panna microdon和Pama pama之间的遗传距离值最高（15.60％），在Otolithoides biauritus和Panna microdon之间的遗传距离最低（0.30％）。研究表明，为了根据核苷酸序列鉴定各个鱼类，可以将细胞色素c氧化酶亚基1（COI）基因用作条形码。