CRISPR-Cas9 technologies have transformed the study of genetic pathways governing cellular differentiation and function. Recent advances have adapted these methods to immune cells, which has accelerated the pace of functional genomics in immunology and enabled new avenues for the design of cellular immunotherapies for cancer. In this review, we summarize recent developments in CRISPR-Cas9 technology and discuss how they have been leveraged to discover and manipulate novel genetic regulators of the immune system. We envision that these results will provide a valuable resource to aid in the design, implementation, and interpretation of CRISPR-Cas9-based screens in immunology and immuno-oncology.

CRISPR-Cas9 技术改变了对控制细胞分化和功能的遗传途径的研究。最近的进展使这些方法适用于免疫细胞，这加快了免疫学中功能基因组学的步伐，并为癌症细胞免疫疗法的设计开辟了新途径。在这篇综述中，我们总结了 CRISPR-Cas9 技术的最新发展，并讨论了如何利用它们来发现和操纵免疫系统的新型遗传调节剂。我们设想这些结果将提供宝贵的资源，以帮助设计、实施和解释免疫学和免疫肿瘤学中基于 CRISPR-Cas9 的筛选。