Clubroot resistance has been introgressed into spring canola from various sources; however, the molecular basis of this using multiple resistances has not yet been investigated. We conducted a comparative transcriptomic study using resistance introgressed from rutabaga (RtR) and winter canola cv. ‘Mendel’ (MdR) and identified ~3500 upregulated genes of which 8–9% genes were involved in primary and secondary metabolic pathways. Treatment of the susceptible plants with trehalose suggested that the modulation of primary metabolism can increase resistance to clubroot. While comparing the DEGs from BnaA08 (RtR) and BnaA03 (MdR), we found that the gene CRF4 could be essential for mediating resistance. We also developed SSR markers from the upregulated genes for use in breeding.

各种根源已将抗根瘤病渗入春油菜籽中。但是，尚未研究使用多重电阻的分子基础。我们使用大头菜（RtR）和冬季双低油菜（canola cv）渗入的抗性进行了比较转录组研究。'Mendel'（MdR）并鉴定出约3500个上调基因，其中8–9％的基因参与一级和二级代谢途径。用海藻糖处理易感植物表明，初级代谢的调节可增加对根肿病的抗性。在比较来自BnaA08（RtR）和BnaA03（MdR）的DEG时，我们发现CRF4基因对于介导抗性可能是必不可少的。我们还从上调的基因中开发了SSR标记，用于育种。