Prawn-plant eco-symbiotic culture system (PECS) is an efficient symbiotic culture system, in which prawn and plants are placed in the same culture space according to a certain proportion. In the study, the potential of PECS to impact microbial communities and metabolites in the intestine of cultured giant river prawn Macrobrachium rosenbergii is assessed. We found that the growth parameters (such as length gain, weight gain and specific growth rate) of PECS with 15 % feed ratio were significantly improved by 4 week culture. And PECS modulated growth profiles of Lactobacillus, Bifidobacterium, Atopobium cluster, and Roseburia subcluster bacteria, all of which increased significantly with 15 % feed ratio in the intestines of M. rosenbergii. Growth of Faecalibacterium prausnitzii, and bacteria referable to the Desulfovibrionales and Desulfuromonadales, were influenced by PECS, whereas growth of Clostridium histolyticum and C. coccoides/Eubacterium rectale groups were not significantly affected. PECS with 15 % feed ratio positively impacted short-chain fatty acids (SCFA) and branched-chain fatty acids (BCFA) production, and reduced lactate levels, without causing any ammonia cytotoxic effects. Well growth performance of the prawn was consistent with increase in intestinal benefit microbiota. In general, PECS (with a feed ratio of 15 %) proved to be beneficial for promoting organic acids and growth performance via modulated intestinal microbiota, indicating PECS with reasonable feed ratio represent a promising alternative to traditional pond monoculture of M. rosenbergii.

大虾植物共生共生系统（PECS）是一种有效的共生培养系统，其中虾和植物按一定比例放置在相同的培养空间中。在这项研究中，评估了PECS影响养殖的大河虾罗氏沼虾肠道微生物群落和代谢产物的潜力。我们发现，通过4周的培养，具有15％进料比的PECS的生长参数（如体长，体重增加和比生长速率）得到了显着改善。PECS调节了乳酸杆菌，双歧杆菌，拟声团簇和玫瑰菌的生长曲线亚群细菌，在罗氏沼虾的肠道中，所有这些都以15％的进料比显着增加。的生长Faecalibacterium prausnitzii和细菌可参照到Desulfovibrionales和Desulfuromonadales，通过PECS的影响，而生长溶组织梭菌和C. coccoides /真杆菌rectale各组均未受到明显影响。进料比为15％的PECS对短链脂肪酸（SCFA）和支链脂肪酸（BCFA）的产生产生积极影响，并降低了乳酸水平，而没有引起任何氨细胞毒性作用。对虾的良好生长性能与肠道有益菌群的增加一致。通常，PECS（进料比例为15％）通过调节肠道菌群被证明有利于促进有机酸和生长性能，这表明具有合理进料比例的PECS可以替代罗氏沼虾的传统池塘单养。