Microbial recurrent infections and antimicrobial resistance have motivated the researchers to explore bioactive compounds as safe alternative antimicrobials to target pathogenic microorganisms. Interest in finding new biologically active substances to replace conventional antibiotics in aquatic feed has rapidly increased, since the misuse or overuse of antibiotics produced antibiotic resistance. Glycerol monolaurate (GML), has attracted attention of researchers due to its growth-promoting and immunomodulatory potential by exerting beneficial effects on the gut microbiota and host health. This study aimed to evaluate the effects of dietary GML using as a feed additive on the growth performance, blood lipid profile, hepatic lipid deposition, body composition, digestive enzymes, serum anti-inflammatory activities, and gut microbiota of zebrafish. The study’s findings showed that diet supplemented with 750 mg kg⁻¹ of GML, significantly improved the growth performance, feed utilization and intestinal lipase activity, as well as body crude lipid content without inducing hepatic fat accumulation. GML supplementation also promoted the serum anti-inflammatory potential by elevating the level of TGF-β1 and IL-10. Moreover, dietary supplementation of GML positively restructured the intestinal microbial ecology by improving the relative abundance of several favorable bacteria, including Cetobacterium, Shewanella, and Vibrio at the genus level. Conclusively, study’s findings suggest that GML supplementation contributes in improving the growth, digestive enzymes activities, anti-inflammatory potential, and gut microbiota, indicating GML promising potential as a feed additive in aquaculture nutrition.

微生物的反复感染和抗药性促使研究人员探索生物活性化合物，将其作为靶向病原微生物的安全替代抗微生物剂。寻找新的生物活性物质替代水产饲料中常规抗生素的兴趣迅速增加，因为滥用或滥用抗生素会产生抗生素耐药性。甘油单月桂酸酯（GML）由于其对肠道菌群和宿主健康产生有益作用而具有促进生长和免疫调节的潜力，因此引起了研究人员的关注。这项研究旨在评估日粮GML作为饲料添加剂对斑马鱼生长性能，血脂谱，肝脂质沉积，身体成分，消化酶，血清抗炎活性和肠道菌群的影响。GML ^-1显着改善了生长性能，饲料利用率和肠道脂肪酶活性，以及​​体内粗脂肪含量，而不会引起肝脏脂肪的积累。补充GML还可以通过提高TGF-β1和IL-10的水平来促进血清抗炎潜能。此外，通过提高几种有益细菌的相对丰度，包括Cetobacterium，Shewanella和Vibrio的相对丰度，GML的膳食补充剂可以积极地重组肠道微生物生态。在属水平上。结论是，研究结果表明，补充GML有助于改善生长，消化酶活性，抗炎潜能和肠道菌群，表明GML有望作为水产养殖营养中的饲料添加剂。