Several secondary metabolites are associated with plant resistance against herbivores. Cassava genotypes present a wide variety of metabolites with insecticidal potential, but little is known about the identity of these compounds. The present work was conducted for the identification of insecticidal molecules present in cassava genotypes. To this purpose, we firstly evaluated the development of the generalist herbivore Spodoptera frugiperda (J.E. Smith, 1797) (Lepidoptera: Noctuidae) fed with leaves from six cassava genotypes (IAC-14, IAC-90, IAC-12, IAC-Caapora, Baianinha and MEcu 72). Following bioassays, we measured the levels of two anti-herbivory related groups of compounds (phenolics and steroidal saponins) in all genotypes using colorimetric methods. The metabolic fingerprinting (GC–MS) of three contrasting cassava genotypes selected by their biological interferences over S. frugiperda was performed. The most unsuitable nutritional indices were observed for larvae fed with MEcu 72. Lower reproductive indices were observed for adults in which larval stages were fed with Baianinha or MEcu 72. Steroidal saponin content was similar in all genotypes, but phenolic content was 25% higher in MEcu 72. GC–MS metabolite fingerprinting of MEcu 72, Baianinha and IAC-Caapora resulted in the annotation of 53 metabolites in which 20 presented different relative abundance among the evaluated genotypes. Molecules such as myo-inositol-2-monophosphate, p-coumaric acid, chlorogenic acid, p-hydroxybenzoic acid, octadecadienoic acid and n-hexacosane were more abundant in MEcu 72 or Baianinha than in IAC-Caapora. The possible roles of these molecules in the development of S. frugiperda are discussed.

几种次生代谢产物与植物对草食动物的抗性有关。木薯基因型表现出多种具有杀虫潜能的代谢物，但对这些化合物的身份知之甚少。目前的工作是为了鉴定木薯基因型中存在的杀虫分子。为此，我们首先评估了多食草食性斜纹夜蛾的发展（JE Smith，1797）（鳞翅目：夜蛾科）饲喂了六种木薯基因型（IAC-14，IAC-90，IAC-12，IAC-Caapora，Baianinha和MEcu 72）的叶子。进行生物测定后，我们使用比色法测量了所有基因型中两个与抗草食动物有关的化合物（酚类和甾体皂苷）的水平。可以通过在生物干扰选得的三种对比木薯基因型代谢指纹图谱（GC-MS）草地夜蛾被执行了。对于饲喂MEcu 72的幼虫，最不适当的营养指数。对于幼虫分给Baianinha或MEcu 72的幼虫，其生殖指数较低。在所有基因型中，类固醇皂苷的含量均相似，但其中酚类含量高25％。 MEcu 72. MEcu 72，Baianinha和IAC-Caapora的GC-MS代谢指纹图谱注释了53种代谢物，其中20种表现出不同的相对丰度。分子，如肌醇-2-单磷酸肌醇，对香豆酸，绿原酸，对羟基苯甲酸，十八碳二烯酸和n-cucosane在MEcu 72或Baianinha中比在IAC-Caapora中更丰富。讨论了这些分子在S. frugiperda发育中的可能作用。