ABSTRACT

The aim of this study was phenotypic and genotypic characterization of antibiotic-resistant food-borne Enterobacterales. The largest number of isolates was identified as Enterobacter cloacae (42.4%) followed by Escherichia coli (9.8%), Proteus mirabilis, Salmonella enterica, Proteus penneri, Citrobacter freundii (7.6% each), Citrobacter braakii (6.6%), Klebsiella pneumoniae and Klebsiella oxytoca (5.4% each). More than half of isolates (52.2%) were resistant to at least one antibiotic. The majority were resistant to amoxicillin-clavulanate (28.3%) and ampicillin (19.5%). ESBL(+) phenotype was showed by 26 isolates and AmpC(+) phenotype by 32 isolates. The bla_CTX-M gene was carried by 53.8% of ESBL-positive isolates, gene from CIT family by 43.8% of AmpC-positive isolates. Our results suggest that more attention should be paid to antibiotic resistance of food-borne Enterobacterales. The presence of transmissible antibiotic resistance markers is an important criterion in the evaluation of food safety.

摘要

本研究的目的是对耐抗生素的食源性肠杆菌进行表型和基因型鉴定。分离株数量最多的是阴沟肠杆菌（42.4%），其次是大肠杆菌（9.8%）、奇异变形杆菌、肠沙门氏菌、变形杆菌、弗氏柠檬酸杆菌（各占 7.6%）、布拉基柠檬酸杆菌（6.6%）、肺炎克雷伯菌和产酸克雷伯菌（各 5.4%）。超过一半的分离株（52.2%）对至少一种抗生素有抗药性。大多数人对阿莫西林-克拉维酸（28.3%）和氨苄青霉素（19.5%）耐药。26 个分离株显示 ESBL(+) 表型，32 个分离株显示 AmpC(+) 表型。这bla _CTX-M基因由 53.8% 的 ESBL 阳性分离物携带，来自 CIT 家族的基因由 43.8% 的 AmpC 阳性分离物携带。我们的研究结果表明，应该更加关注食源性肠杆菌的抗生素耐药性。是否存在可传播的抗生素抗性标记是评价食品安全的一个重要标准。