Liquid-assisted mechanochemistry as a versatile approach for the coupling of a nucleoside phosphoramidite with a 5′-OH partially protected nucleoside has been investigated. Noted advantages over reported methods were a simplified reaction protocol, a drastic reduction in the use of toxic solvents, the facilitation of mechanochemical reactions through the improved mixing of solid reagents, and low hydrolytic product formation.

中文翻译：

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通过机械化学亚磷酰胺化学合成核苷酸二聚体的研究

已经研究了液体辅助机械化学作为将核苷亚磷酰胺与5'-OH部分保护的核苷偶联的通用方法。与已报道的方法相比，其显着优势是简化了反应流程，大大减少了有毒溶剂的使用，通过改进固体试剂的混合促进了机械化学反应，并降低了水解产物的形成。