This study aims the reproductive and DNA content characterization of ten different Paspalum species from the Plicatula group (P. atratum, P. compressifolium, P. guenoarum, P. lenticulare, P. lepton, P. oteroi, P. plicatulum, P. rhodopedum, P. rojasii and P. wrightii), contributing to the determination of the best breeding and conservation method to be used. The DNA content of 150 accessions was estimated by flow cytometry using foliar tissues. The reproductive mode of 135 accessions was characterized by flow cytometric seed screening, and five accessions were determined by cytoembryological ovary analysis. DNA content ranged from 1.50 pg (BGP 380) to 4.27 pg (BGP 193), both from P. compressifolium species. Most of the accessions were compatible to the tetraploid level, except for BGP 281 (P. lenticulare), BGP 272 (P. rojasii), and BGP 380 (P. compressifolium), which were diploids. Accessions with tetraploid DNA content presented apomictic reproduction, whereas the three diploid accessions were sexual. These results contribute to the reproductive knowledge of species of the Plicatula group. The diploid sexual accessions identified represent important material to overcome the apomictic barrier in crosses and can be used after polyploidization as female parents in crosses with apomictic accessions in Paspalum breeding programs.

这项研究的目的是从Picaatula组（P. atratum，P. compressifolium，P. guenoarum，P. lenticulare，P. lepton，P. oteroi，P. plicatulum，P.rhopedpedum）的十种不同的Paspalum物种的生殖和DNA含量表征。 （P. rojasii和P. wrightii），有助于确定最佳的育种和保存方法。通过使用叶面组织的流式细胞仪评估了150个种质的DNA含量。通过流式细胞术种子筛选鉴定了135个种质的生殖方式，并通过细胞胚卵巢分析确定了5个种质。DNA含量范围从1.50 pg（BGP 380）到4.27 pg（BGP 193），均来自于compress.ol。除BGP 281（P. lenticulare），BGP 272（P. rojasii）和BGP 380（P. compressifolium），是二倍体。具有四倍体DNA含量的种质表现出无融合生殖繁殖，而三个二倍体种质是有性的。这些结果有助于Plicatula组物种的生殖知识。鉴定出的二倍体有性种质是克服杂交中无融合生殖障碍的重要材料，可在多倍体化后作为雌性亲本与Paspalum育种计划中的无融合生殖种质一起使用。