Agricultural airborne work exposures are complex in nature and workplace exposures are a risk for respiratory outcomes in workers. Endotoxin and glyphosate are two common agents in agricultural exposures. While endotoxin (lipopolysaccaride, LPS) is a potent inflammatory agent it explains only a portion of the respiratory inflammatory response. The inflammatory potential when LPS is presented with another common agricultural respiratory agent, glyphosate, is not known. Methods: Mice were assigned to four treatment groups: control, LPS alone, glyphosate alone, glyphosate and LPS combined. Treatments were for 1, 5 or 10 days. Results: Five days of repeated exposure to the comintation of LPS and glyphosate resulted in higher neutrophil counts, myloperoxidase, TNF-α, IL-6, KC levels, and ICAM-1 and TLR-2 expression compared to the same length of treatment to LPS or glyphosate alone. After 10-days of exposure, inflammatory responses decreased, however leukocyte infiltration persisted along with increases in IL-4. Conclusions: Glyphosate exposure modified LPS induced lung inflammatory responses and TLR-2 may be important in the modulated inflammatory response.

农业空气工作暴露本质上很复杂，工作场所暴露是工人呼吸系统疾病的风险。内毒素和草甘膦是农业接触中的两种常见物质。虽然内毒素（脂多糖，LPS）是一种有效的炎症介质，但它只能解释部分呼吸道炎症反应。当 LPS 与另一种常见的农业呼吸剂草甘膦一起出现时，潜在的炎症尚不清楚。方法：将小鼠分为四个治疗组：对照组、单独使用 LPS、单独使用草甘膦、草甘膦和 LPS 联合治疗组。治疗持续1、5或10天。结果：与相同时间的治疗相比，重复暴露于 LPS 和草甘膦的组合五天导致中性粒细胞计数、髓过氧化物酶、TNF-α、IL-6、KC 水平以及 ICAM-1 和 TLR-2 表达更高。单独使用 LPS 或草甘膦。暴露 10 天后，炎症反应减少，但白细胞浸润持续存在，IL-4 增加。结论：草甘膦暴露修饰的 LPS 诱导肺部炎症反应，TLR-2 可能在调节炎症反应中发挥重要作用。