The optical absorption, fluorescence, phosphorescence in UV and visible spectral range, and effect of light irradiation on spectral properties of DNA with the presence of nickel ions are studied. The quantity of nickel ions varies from 1 ion per 500 base pairs of DNA to 2 ions per base pair. Three important features fixed: the shape of fluorescence and phosphorescence spectra of the DNA do not change in presence of Ni, but their intensity depends on the number of nickel ions in solution; phosphorescence intensity decreases more rapidly than fluorescence; the small decrease of DNA photodegradation rate in presence of Ni is observed. The average of triplet exciton path length evaluated from the dependence of phosphorescence/fluorescence intensity ratio on the relative concentration of nickel ions is 30–20 DNA pair sequence length. It was proposed that nickel ions interact in the outer side of DNA with the phosphate groups and do not penetrate intra macromolecular space.

中文翻译：

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镍离子对DNA光谱特性和光稳定性的影响

研究了在存在镍离子的情况下在紫外和可见光谱范围内的光吸收，荧光，磷光，以及光辐照对DNA光谱特性的影响。镍离子的数量从DNA每500个碱基对的1个离子到每碱基对2个离子的不等。已修复的三个重要特征是：在镍的存在下，DNA的荧光和磷光光谱的形状不会改变，但其强度取决于溶液中镍离子的数量。磷光强度的下降比荧光强度下降更快；观察到在Ni存在下DNA光降解速率的小幅下降。根据磷光/荧光强度比对镍离子相对浓度的依赖性评估的三重态激子路径长度的平均值为30–20 DNA对序列长度。