Abstract

The removal of textile dyes from wastewater by using plant peroxidases (PODs) offers environmentally effective solutions. Therefore, researching the availability and applicability of PODs from different sources becomes important area for the sustainable process design. In this article, PODs were produced in vitro from white and red cabbage callus cultures for evaluating their potential usage to decolourization of the Reactive Blue 19 (RB-19) and Acid Blue 25 (AB-25) textile dyes for the first time. The PODs with high activity were purified 133-fold with a specific activity of 45,083 U mg⁻¹ and 134-fold with a specific activity of 26,785 U mg⁻¹ from produced white and red cabbage calluses by single-step affinity chromatography. The molecular weights of purified red cabbage callus peroxidase (r-POD) and white cabbage callus peroxidase (w-POD) were found to be as 43 and 44 kDa, respectively. In the assays to determine the r-POD and w-POD dye decolourization potential, noteworthy degradation levels were recorded for RB-19 (>85) and AB-25 (>88) dyes in the reactions catalysed by w-POD. Also, r-POD exhibited maximum decolourization efficiency of % 68 for RB-19 and %73 for AB-25 dyes under the conditions at 50 °C, pH 5.5 and in the presence of 4 mM H₂O₂.

摘要

通过使用植物过氧化物酶 (POD) 从废水中去除纺织染料提供了对环境有效的解决方案。因此，研究不同来源 POD 的可用性和适用性成为可持续工艺设计的重要领域。在这篇文章中，PODs 是从白色和红色卷心菜愈伤组织培养物中首次生产的，用于评估它们在活性蓝 19 (RB-19) 和酸性蓝 25 (AB-25) 纺织染料脱色方面的潜在用途。具有高活性的 POD 被纯化 133 倍，比活性为 45,083 U mg ^-1和 134 倍，比活性为 26,785 U mg ^-1通过单步亲和色谱法从产生的白色和红色卷心菜愈伤组织中提取。纯化的红甘蓝愈伤组织过氧化物酶 (r-POD) 和白甘蓝愈伤组织过氧化物酶 (w-POD) 的分子量分别为 43 和 44 kDa。在确定 r-POD 和 w-POD 染料脱色潜力的测定中，在 w-POD 催化的反应中记录了 RB-19 (>85) 和 AB-25 (>88) 染料的显着降解水平。_{此外，在 50 °C、pH 5.5 和 4 mM H 2} O ₂存在的条件下，r-POD 对 RB-19 和 AB-25 染料的最大脱色效率分别为 % 68 和 % 73 。