ABSTRACT

Objective

Ginger (Zingiber officinale Roscoe) is considered to be one of the most commonly consumed dietary condiments of the world. The present study was designed to explicate the protective role of zingerone; an active ingredient of ginger in complete Freund’s adjuvant (FCA)-immunized arthritic rats.

Methods

24 Wistar rats were divided into 4 groups with 6 rats each. Group I as control followed by group II, III and IV were treated with single intradermal injection of FCA (0.1 ml = 100 µg) to induce rheumatoid arthritis. Group III and IV were also administered with zingerone orally at 25 mg/kg b.w for 3 weeks at two different time points.

Results

Adjuvant-treated rats exhibited a significant increase in lipid peroxidation and a reduction in the enzymatic antioxidants such as SOD, catalase and GPx, in the liver and joint tissues. Moreover, FCA inoculation resulted in the increase in levels of NF-κB, TGF-β, TNF-α, IL-1β, IL-6 and Hs-CRP and a decrease in IL-10 levels. Zingerone significantly reduced the levels of NF-κB, TGF-β, TNF-α, IL-1β, IL-6 and Hs-CRP and markedly increased IL-10 levels. Levels of antioxidant enzymes were also restored by zingerone treatment.

Discussion

Oral administration of zingerone ameliorated inflammatory outburst and decreased oxidative stress, suggesting its role in the prevention of rheumatoid arthritis. Further mechanistic insights are necessary to study the exact mechanism involved.

中文翻译：

---

Zingerone (4-(四-羟基-3-甲基苯基)丁烷-二-1) 通过调节炎症细胞因子和抗氧化剂来调节佐剂诱导的类风湿性关节炎

摘要

客观的

生姜（Zingiber officinale Roscoe）被认为是世界上最常食用的膳食调味品之一。本研究旨在阐明姜油酮的保护作用；完全弗氏佐剂（FCA）免疫关节炎大鼠中生姜的活性成分。

方法

将24只Wistar大鼠分为4组，每组6只。I 组作为对照，随后 II、III 和 IV 组通过单次皮内注射 FCA（0.1 ml = 100 µg）来诱导类风湿性关节炎。III组和IV组还在两个不同时间点以25mg/kg体重口服姜酮，持续3周。

结果

佐剂治疗的大鼠肝脏和关节组织中脂质过氧化显着增加，酶促抗氧化剂（如 SOD、过氧化氢酶和 GPx）减少。此外，FCA接种导致NF-κB、TGF-β、TNF-α、IL-1β、IL-6和Hs-CRP水平升高，而IL-10水平降低。Zingerone 显着降低 NF-κB、TGF-β、TNF-α、IL-1β、IL-6 和 Hs-CRP 的水平，并显着增加 IL-10 的水平。姜油酮治疗也恢复了抗氧化酶的水平。

讨论

口服姜油酮可改善炎症爆发并减少氧化应激，表明其在预防类风湿性关节炎中的作用。为了研究所涉及的确切机制，需要进一步的机制见解。