Background & objectives: Dengue and chikungunya are two mosquito-borne viral diseases transmitted by Aedes mosquito species and are a great public health concern in India. The present study was aimed to check the influence of antagonistic crustaceans, especially Mesocyclops aspericornis and Daphnia magna on Ae. aegypti L. mosquito population.
Method: Variable ratios of these crustaceans (Aedes: Mesocyclops: Daphnia) against Ae. aegypti larvae were tested by putting them in plastic beakers having dechlorinated water along with yeast stock solution provided as food, and kept in BOD incubator at a temperature of 26 ±1°C.
Results: Out of all tested concentrations, 1:1:3 where the number of D. magna was thrice the number of Mesocyclops and Aedes; larvae showed a significant delay of 5–6 days in the developmental period. Maximum reduction in the emergence of females was recorded in the ratio 1:1:3, i.e. only 6.5 ± 0.47 females emerged when Daphnia used thrice the number of Aedes larvae. Body size of both males and females emerged from treated sets was found to be significantly reduced. The longevity of adults was also reduced from 8–17 days to 5–8 days in the case of males and from 14–26 days to 5–9 days in females.
Interpretation & conclusion: Among variable ratios tested under laboratory conditions, 1:1:2 and 1:1:3 ratios were found to be the effective ratios that greatly reduced the development duration, survivorship of larvae, and the number of larvae emerging into adulthood. Thus, antagonistic crustaceans specifically Mesocyclops and Daphnia can be used as biocontrol agents for the sustainable control of container breeding mosquitoes.

中文翻译：

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实验室条件下拮抗甲壳类动物对埃及伊蚊幼虫种群的影响

背景与目标：登革热和基孔肯雅热是由伊蚊传播的两种蚊媒病毒性疾病，是印度重大的公共卫生问题。本研究旨在检查拮抗甲壳类动物，特别是Mesocyclops aspericornis和Daphnia magna对Ae 的影响。aegypti L. 蚊子种群。
方法：这些甲壳类动物（伊蚊：中环目动物：水蚤）对Ae 的可变比率。埃及伊蚊幼虫通过将它们与作为食物提供的酵母原液一起放入装有脱氯水的塑料烧杯中进行测试，并保存在温度为 26±1°C 的 BOD 培养箱中。
结果：在所有测试的浓度中，1:1:3，其中D. magna的数量是Mesocyclops和Aedes数量的三倍；幼虫在发育期显示显着延迟 5-6 天。雌性出现的最大减少记录为 1:1:3 的比例，即。当水蚤使用三倍于伊蚊幼虫的数量时，只有 6.5 ± 0.47 只雌性出现。发现从处理组中出现的雄性和雌性的体型显着减小。成年雄性的寿命也从 8-17 天减少到 5-8 天，雌性从 14-26 天减少到 5-9 天。
解读与结论：在实验室条件下测试的可变比率中，发现 1:1:2 和 1:1:3 的比率是大大减少发育持续时间、幼虫存活率和进入成年期的幼虫数量的有效比率。因此，拮抗甲壳类动物，特别是Mesocyclops和水蚤可以用作生物防治剂，用于可持续控制容器繁殖蚊子。