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Potato virus Y isolates infecting bell pepper from parts of Southern Africa display distinct recombination patterns
Physiological and Molecular Plant Pathology ( IF 2.8 ) Pub Date : 2021-03-30 , DOI: 10.1016/j.pmpp.2021.101638
Charles Karavina , Jacques Davy Ibaba , Augustine Gubba

Potato virus Y (PVY) infects economically important solanaceous crops in Zimbabwe. However, there is little information on the genome sequences of isolates occurring in this country. In this study, RNASeq of PVY-infected pepper leaves that were sampled from different locations in Zimbabwe was performed. The PVY genome sequences recovered by de novo assembly of the generated reads were checked for recombination patterns before phylogenetic analyses. Three PVY isolates: d7-Bindura, prime-e5, and q19-Harare were all found to have arisen from a single recombination event involving PVYO and PVYC strains. Phylogenetic analyses provided further evidence of recombination. The isolates in this study were grouped with a pepper-infecting PVY isolate previously found in South Africa. More studies of this kind need to be conducted in Africa to determine the outspread of this particular recombinant virus.



中文翻译:

马铃薯病毒Y分离株感染南部非洲部分地区的灯笼椒,表现出独特的重组模式

马铃薯Y(PVY)病毒感染津巴布韦具有重要经济意义的茄科作物。但是,在这个国家,关于分离株的基因组序列的信息很少。在这项研究中,进行了从津巴布韦不同地点采样的PVY感染辣椒叶的RNASeq。在系统发育分析之前,检查通过重新组装产生的读数的PVY基因组序列的重组模式。发现三个PVY分离株:d7-Bindura,prime-e5和q19-Harare均来自涉及PVY O和PVY C的单个重组事件株。系统发育分析提供了重组的进一步证据。本研究中的分离株与以前在南非发现的辣椒感染PVY分离株分组。需要在非洲进行更多此类研究,以确定这种特定重组病毒的扩散。

更新日期:2021-04-08
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