The current study investigated associations between variation in the bovine perilipin-2 gene (PLIN2) and milk traits (milk fat content, milk protein content, milk yield and milk fatty acid (FA) component levels) in 409 New Zealand pasture-grazed Holstein-Friesian × Jersey-cross (HF × J-cross or Kiwicross™) cows. Five nucleotide sequence variants were found in three regions of the gene, including c.17C>T in exon 2, c.53A>G in exon 3, c.595+23G>A and c.595+104_595+108del in intron 5, and c.*302T>C in the 3′-untranslated region. The c.*302T>C substitution produces two nucleotide sequence variants (A5 and B5), and this variation was associated with variation in milk protein content and milkfat composition for C10:0, C11:0, C12:0, C13:0 and C16:0 FA and medium-chain fatty acid (MCFA) and long-chain fatty acid (LCFA) groups. After correcting for the effect of variation in the diacylglycerol acyl-CoA acyltransferase 1 gene (DGAT1) that results in the amino acid substitution p.K232A, variation in the FA binding protein 4 gene (FABP4) and variation in the stearoyl-CoA desaturase (Δ-9-desaturase) gene (SCD) that results in the amino acids substitution p.A293V, significant differences between A5A5 and B5B5 cows were found for C10:0, C11:0, C12:0, C13:0, C16:0, and the MCFA, LCFA, total saturated FA and C10:1 index groups. This suggests that nucleotide sequence variation in PLIN2 may be affecting milk FA component levels.

中文翻译：

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PLIN2的变异及其与奶牛乳性状和乳脂组成的关系

目前的研究调查了牛perilipin-2基因变异之间的关联。PLIN2) 和 409 头新西兰牧场放牧的 Holstein-Friesian × Jersey-cross (HF × J-cross or Kiwicross™) 奶牛的牛奶性状（牛奶脂肪含量、牛奶蛋白质含量、牛奶产量和牛奶脂肪酸 (FA) 成分水平） . 在基因的三个区域发现了5个核苷酸序列变异，包括外显子2的c.17C>T、外显子3的c.53A>G、内含子5的c.595+23G>A和c.595+104_595+108del , 和 c.*302T>C 在 3'-非翻译区。c.*302T>C 替换产生两个核苷酸序列变体 (一种5和乙5)，这种变化与 C10:0、C11:0、C12:0、C13:0 和 C16:0 FA 以及中链脂肪酸 (MCFA) 和长链脂肪酸的乳蛋白含量和乳脂组成的变化有关脂肪酸 (LCFA) 基团。在校正二酰基甘油酰基辅酶A酰基转移酶1基因变异的影响后（DGAT1) 导致氨基酸取代 p.K232A，FA 结合蛋白 4 基因的变异 (FABP4）和硬脂酰辅酶A去饱和酶（Δ-9-去饱和酶）基因的变异（SCD) 导致氨基酸取代 p.A293V，之间的显着差异一种5一种5和乙5乙5在 C10:0、C11:0、C12:0、C13:0、C16:0 和 MCFA、LCFA、总饱和 FA 和 C10:1 指数组中发现了奶牛。这表明核苷酸序列变异PLIN2可能会影响牛奶 FA 成分水平。