The goal of this study is to understand the ability of a newly developed barrier membrane to enhance bone tissue regeneration. Here in this study we present the in vitro characterization of the barrier membrane made from type I collagen and crosslinked by oligomeric proanthocyanidins (OPCs). The effects of the membrane (P-C film) on cell cycle, proliferation, alkaline phosphatase activity, and mineralization were evaluated using the human osteoblast cell line MG-63, while the barrier ability was examined using MG-63 cells, as well as the human skin fibroblast cell line WS-1. The pore size is one of the factors that plays a key role in tissue regeneration, therefore, we evaluated the pore size of the membrane using a capillary flow porometer. Our results showed that the mean pore size of the P-C film was approximately 7–9 µm, the size known to inhibit cell migration across the membrane. The P-C film also demonstrated excellent cell viability and good biocompatibility, since the cell number increased with time, with MG-63 cells proliferating faster on the P-C film than in the cell culture flask. Furthermore, the P-C film promoted osteoblast differentiation, resulting in higher alkaline phosphatase activity and mineralization. Therefore, our results suggest that this P-C film has a great potential to be used in guided bone regeneration during periodontal regeneration and bone tissue engineering.

这项研究的目的是了解新开发的屏障膜增强骨组织再生的能力。在这项研究中，我们展示了由 I 型胶原蛋白制成并由低聚原花青素 (OPCs) 交联的屏障膜的体外表征。使用人成骨细胞系 MG-63 评估膜（PC 膜）对细胞周期、增殖、碱性磷酸酶活性和矿化的影响，同时使用 MG-63 细胞和人皮肤成纤维细胞系 WS-1。孔径是在组织再生中起关键作用的因素之一，因此，我们使用毛细管流量计评估了膜的孔径。我们的结果表明 PC 薄膜的平均孔径约为 7-9 µm，已知抑制细胞跨膜迁移的大小。PC 膜还表现出优异的细胞活力和良好的生物相容性，因为细胞数量随时间增加，PC 膜上的 MG-63 细胞增殖速度比细胞培养瓶中的快。此外，PC 膜促进成骨细胞分化，导致更高的碱性磷酸酶活性和矿化。因此，我们的研究结果表明，这种 PC 膜在牙周再生和骨组织工程过程中用于引导骨再生方面具有很大的潜力。PC 膜促进成骨细胞分化，导致更高的碱性磷酸酶活性和矿化。因此，我们的研究结果表明，这种 PC 膜在牙周再生和骨组织工程过程中用于引导骨再生方面具有很大的潜力。PC 膜促进成骨细胞分化，导致更高的碱性磷酸酶活性和矿化。因此，我们的研究结果表明，这种 PC 膜在牙周再生和骨组织工程过程中用于引导骨再生方面具有很大的潜力。