The abnormal proliferation of vascular smooth muscle cells (VSMCs) is crucial in the atherosclerosis. Although long noncoding RNAs (lncRNAs) are implicated in a variety of diseases, their roles in activation of VSMCs proliferation and vascular disorder diseases are not well understood. In addition, heterogeneous nuclear ribonucleoprotein A2/B1 (hnRNPA2/B1) was reported to participate in lncRNAs-mediated function. Herein, we propose to investigate the role of lncRNA AC105942.1 and hnRNPA2/B1 in pathological VSMCs proliferation and the possible mechanisms in vitro. We have identified that lncRNA AC105942.1 was downregulated and hnRNPA2/B1 was upregulated in atherosclerotic plaques compared with normal artery tissues. Enhanced lncRNA AC105942.1 could noticeably inhibit Ang II-induced VSMCs proliferation. Further investigation suggested that lncRNA AC105942.1 could downregulate the expression of hnRNPA2/B1 and then regulate the level of CDK4 and p27. Taken together, our study indicated that lncRNA AC105942.1 downregulated hnRNPA2B1 to protect against the atherosclerosis by suppressing VSMCs proliferation. LncRNA AC105942.1 and hnRNPA2/B1 could represent potential therapeutic and diagnostic targets to atherosclerosis-related diseases.

中文翻译：

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LncRNA AC105942.1下调hnRNPA2 / B1以减轻血管平滑肌细胞的增殖

血管平滑肌细胞（VSMC）的异常增殖在动脉粥样硬化中至关重要。尽管长的非编码RNA（lncRNA）与多种疾病有关，但它们在激活VSMC增殖和血管疾病中的作用尚不十分清楚。另外，据报道异质核糖核蛋白A2 / B1（hnRNPA2 / B1）参与lncRNAs介导的功能。在这里，我们建议调查lncRNA AC105942.1和hnRNPA2 / B1在病理性VSMCs增殖中的作用及其可能的体外机制。我们已经发现，与正常动脉组织相比，动脉粥样硬化斑块中的lncRNA AC105942.1被下调，而hnRNPA2 / B1被上调。增强的lncRNA AC105942.1可以显着抑制Ang II诱导的VSMCs增殖。进一步的研究表明，lncRNA AC105942.1可以下调hnRNPA2 / B1的表达，然后调节CDK4和p27的水平。综上所述，我们的研究表明lncRNA AC105942.1下调了hnRNPA2B1，以通过抑制VSMC增殖来预防动脉粥样硬化。LncRNA AC105942.1和hnRNPA2 / B1可能代表动脉粥样硬化相关疾病的潜在治疗和诊断目标。