In the present study, mupirocin (MP), an antimicrobial agent, was formulated as a nanostructured lipid carrier (NLC) by using a novel method named as melt emulsion ultrafiltration method. For the formulation of NLC, glyceryl monostearate and watermelon seed oil were used as solid and liquid lipids, respectively. The method was optimized for various parameters by Taguchi design of experiment and prepared NLCs were characterized for particle size, polydispersity index (PDI), shape, zeta potential, % drug loading, and in vitro release profile. The optimized NLCs were found to be smooth, monodisperse with PDI 0.229 ± 0.093. NLCs were found to have an average particle size of 139 ± 0.75 nm and +21.9 ± 0.98 mV as zeta potential. The % drug loading of optimized NLCs was found to be 59% ± 0.13%. The optimized NLCs were able to release the drug up to 24 h. The release kinetic study revealed mixed-order kinetics. Hence, it was concluded that the novel method is simple and able to fabricate MP-loaded NLCs with sustained release property and being stable in terms of particle size, PDI, and % drug loading.

中文翻译：

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莫匹罗星纳米结构脂质载体的制备及其体外表征

在本研究中，莫匹罗星 (MP) 是一种抗菌剂，通过使用称为熔体乳液超滤法的新方法配制成纳米结构脂质载体 (NLC)。对于 NLC 的配方，单硬脂酸甘油酯和西瓜籽油分别用作固体和液体脂质。该方法通过田口实验设计针对各种参数进行了优化，制备的 NLC 具有粒度、多分散指数 (PDI)、形状、zeta 电位、药物负载百分比和体外释放曲线的特征。发现优化的 NLC 是光滑的、单分散的，PDI 为 0.229 ± 0.093。发现 NLC 的平均粒径为 139 ± 0.75 nm，Zeta 电位为 +21.9 ± 0.98 mV。发现优化的 NLC 的载药量百分比为 59% ± 0.13%。优化的 NLC 能够释放药物长达 24 小时。释放动力学研究揭示了混合级动力学。因此，得出的结论是，该新方法简单且能够制造具有持续释放特性且在粒径、PDI 和药物负载百分比方面稳定的 MP 负载 NLC。