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Evaluation and genome-wide association study of resistance to bacterial blight race 18 in U.S. Upland cotton germplasm
Molecular Genetics and Genomics ( IF 3.1 ) Pub Date : 2021-03-29 , DOI: 10.1007/s00438-021-01779-w
Hanan Elassbli , Abdelraheem Abdelraheem , Yi Zhu , Zonghua Teng , Terry A. Wheeler , Vasu Kuraparthy , Lori Hinze , David M. Stelly , Tom Wedegaertner , Jinfa Zhang

Bacterial blight (BB), caused by Xanthomonas citri pv. malvacearum (Xcm), is a destructive disease to cotton production in many countries. In the U.S., Xcm race 18 is the most virulent and widespread race and can cause serious yield losses. Planting BB-resistant cotton cultivars is the most effective method of controlling this disease. In this study, 335 U.S. Upland cotton accessions were evaluated for resistance to race 18 using artificial inoculations by scratching cotyledons on an individual plant basis in a greenhouse. The analysis of variance detected significant genotypic variation in disease incidence, and 50 accessions were resistant including 38 lines with no symptoms on either cotyledons or true leaves. Many of the resistant lines were developed in the MAR (multi-adversity resistance) breeding program at Texas A&M University, whereas others were developed before race 18 was first reported in the U.S. in 1973, suggesting a broad base of resistance to race 18. A genome-wide association study (GWAS) based on 26,301 single nucleotide polymorphic (SNP) markers detected 11 quantitative trait loci (QTL) anchored by 79 SNPs, including three QTL on each of the three chromosomes A01, A05 and D02, and one QTL on each of D08 and D10. This study has identified a set of obsolete Upland germplasm with resistance to race 18 and specific chromosomal regions delineated by SNPs for resistance. The results will assist in breeding cotton for BB resistance and facilitate further genomic studies in fine mapping resistance genes to enhance the understanding of the genetic basis of BB resistance in cotton.



中文翻译:

美国陆地棉种质对18号枯萎病抗性的评估和全基因组关联研究

细菌性疫病(BB),由Xanthomonas citri  pv引起。 malvacearum(Xcm)是许多国家/地区对棉花生产的破坏性疾病。在美国,Xcm第18场比赛是最致命,最广泛的比赛,可能会导致严重的产量损失。种植抗BB的棉花品种是控制这种病害的最有效方法。在这项研究中,使用人工接种方法,通过在温室中以单个植物为基础刮擦子叶,评估了335种美国陆地棉品种对18种的抗性。方差分析检测到疾病发生率的显着基因型变异,有50种抗性,包括38株子叶或真叶无症状的品系。许多抗性品系是在德克萨斯A&M大学的MAR(多逆境抗性)育种计划中开发的,而其他抗性品系是在美国首次报道第18种族之前开发的。S.于1973年提出,表明其对18号种族具有广泛的抵抗力。一项基于26,301个单核苷酸多态性(SNP)标记的全基因组关联研究(GWAS)检测到11个由79个SNP锚定的数量性状基因座(QTL),包括三个QTL在三个染色体A01,A05和D02的每个上都有一个QTL,在D08和D10的每个染色体上都有一个QTL。这项研究已经确定了一组对18号种族具有抗性的过时的高地种质,以及由SNP划定的抗性的特定染色体区域。该结果将有助于棉花抗BB的育种,并有助于在精细定位抗性基因中进行进一步的基因组研究,以增进对棉花BB抗性遗传基础的了解。301个单核苷酸多态性(SNP)标记检测了由79个SNP锚定的11个数量性状基因座(QTL),包括三个A01,A05和D02染色体上的每个QTL,以及D08和D10各个染色体上的一个QTL。这项研究已经确定了一组对18号种族具有抗性的过时的高地种质,以及由SNP划定的抗性的特定染色体区域。该结果将有助于棉花抗BB的育种,并有助于在精细定位抗性基因中进行进一步的基因组研究,以增进对棉花BB抗性遗传基础的了解。301个单核苷酸多态性(SNP)标记检测了由79个SNP锚定的11个数量性状基因座(QTL),包括三个A01,A05和D02染色体上的每个QTL,以及D08和D10各个染色体上的一个QTL。这项研究已经确定了一组对18号种族具有抗性的过时的高地种质,以及由SNP划定的抗性的特定染色体区域。该结果将有助于棉花抗BB的育种,并有助于在精细定位抗性基因中进行进一步的基因组研究,以增进对棉花BB抗性遗传基础的了解。这项研究已经确定了一组对18号种族具有抗性的过时的高地种质,以及由SNP划定的抗性的特定染色体区域。该结果将有助于棉花抗BB的育种,并有助于在精细定位抗性基因中进行进一步的基因组研究,以增进对棉花BB抗性遗传基础的了解。这项研究已经确定了一组对18号种族具有抗性的过时的高地种质,以及由SNP划定的抗性的特定染色体区域。该结果将有助于棉花抗BB的育种,并有助于在精细定位抗性基因中进行进一步的基因组研究,以增进对棉花BB抗性遗传基础的了解。

更新日期:2021-03-29
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