Increasing some industrial activities has been constantly contaminated the soil with different toxic metals leading to serious environmental concerns. However, some beneficial soil microorganisms would be useful tools for reducing toxic effects of heavy metals without producing toxic residuals. Therefore, present study was conducted to evaluate potential role of Pseudomonas fluorescens and arbuscular mycorrhizal fungi (AMF) in reducing cadmium toxicity in the Arizona cypress seedlings both individually and in combination. Factorial experiment was conducted based on a completely randomized design with three factors comprising AMF inoculation (Rhizophagus irregularis or Funneliformis mosseae or a mixture of both species, and non-inoculated controls), bacterial inoculation (P. fluorescens or Non-inoculated), and five levels of Cd (0, 5, 10, 15, and 20 mg kg⁻¹) using three replications. Results of this study showed that, Arizona cypress growth decreased when exposed to high levels of Cd contamination. However, co-inoculation plants with all mycorrhizae and P. fluorescens grown under the highest levels of Cd contamination, showed increased photosynthetic pigments. Furthermore, AMF and P. fluorescens increased ascorbate peroxidase activity, which is responsible for the elimination of H₂O₂ when plants exposed to Cd stress. Moreover, high levels of Cd contamination negatively influenced phosphorus uptake by the plants, especially in control plants. Moreover, in seedlings inoculated with mycorrhizal fungi, accumulation of Cd was found to be less, apparently as a result of AMF modification of the influx of Cd into the host plants, as confirmed by reduced hydrogen peroxide and enhanced antioxidant enzyme activities compared to the control plants. AMF treatments increased glomalin related soil proteins concentration compared to the control plants. Also, the highest amount of glomalin production was observed in two species of Mix and FM. In conclusion, either co-inoculation with AMF and P. fluorescens or individual inoculation with AMF could potentially ameliorate harmful effects of Cd on Arizona cypress growth. .

越来越多的工业活动不断地用不同的有毒金属污染土壤，导致严重的环境问题。但是，一些有益的土壤微生物将是减少重金属的毒性作用而又不产生毒性残留物的有用工具。因此，本研究旨在评估荧光假单胞菌和丛枝菌根真菌（AMF）在降低亚利桑那柏树幼苗中镉毒性方面的潜在作用，无论是单独使用还是联合使用。在完全随机设计的基础上进行了析因实验，其中包括接种AMF的三个因素（不规则根瘤菌（Rhizophagus Regularis）或Funneliformis mosseae或两者的混合物，以及未接种的对照），细菌接种（P. fluorescens或未接种）和五种水平的Cd（0、5、10、15和20 mg kg ^-1），重复三次。这项研究的结果表明，当暴露于高水平的Cd污染时，亚利桑那柏的生长会降低。但是，在最高的Cd污染下生长的所有菌根和荧光假丝酵母的共接种植物显示出增加的光合色素。此外，AMF和荧光假单胞菌提高了抗坏血酸过氧化物酶的活性，这有助于消除H ₂ O ₂当植物暴露于镉胁迫下时。此外，高水平的Cd污染对植物的磷吸收有负面影响，特别是在对照植物中。此外，在接种了菌根真菌的幼苗中，发现Cd的积累较少，这显然是由于AMF修饰了进入宿主植物的Cd流入量所致，与对照相比，过氧化氢的减少和抗氧化酶活性的增强证实了这一点。植物。与对照植物相比，AMF处理增加了与草甘膦相关的土壤蛋白质浓度。同样，在混合和FM的两种物种中观察到了最高的gloomalin产生量。总之，可以与AMF和荧光假单胞菌一起接种或单独接种AMF可能会减轻Cd对亚利桑那柏生长的有害影响。。