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A newly established canine NK-type cell line and its cytotoxic properties
Veterinary and Comparative Oncology ( IF 2.3 ) Pub Date : 2021-03-27 , DOI: 10.1111/vco.12695
Malgorzata Grudzien 1 , Aleksandra Pawlak 2 , Justyna Kutkowska 1 , Ewa Ziolo 1 , Edyta Wysokińska 1 , Wojciech Hildebrand 3 , Bożena Obmińska-Mrukowicz 2 , Leon Strzadala 1 , Andrzej Rapak 1
Affiliation  

We established a canine natural killer (NK)-type cell line called CNK-89 derived from a dog with NK cell neoplasia. Immunophenotyping analysis showed positive staining for CD5, CD8, CD45, CD56, CD79a and NKp46, while negative for CD3, CD4, CD14, CD20, CD21, CD34, Thy1, IgG, IgM and MHCII. Polymerase chain reaction analysis revealed the presence of CD56, NKG2D, NKp30, NKp44, NKp46 and perforin, but the absence of CD16, Ly49 and granzyme B mRNA. Treating CNK-89 cells with IL-2 did not change the expression of activating receptors, TNFα and IFNγ secretion and cytotoxic activity, however, treatment with IL-12 alone or in combinations with IL-15, IL-18 and IL-21 caused an increase in granzyme B and CD16 mRNA, IFNγ secretion and cytotoxic properties of the CNK-89 cell line.

中文翻译:

一种新建立的犬NK型细胞系及其细胞毒特性

我们建立了一种名为 CNK-89 的犬类自然杀伤 (NK) 型细胞系,该细胞系来源于患有 NK 细胞瘤形成的狗。免疫表型分析显示 CD5、CD8、CD45、CD56、CD79a 和 NKp46 染色呈阳性,而 CD3、CD4、CD14、CD20、CD21、CD34、Thy1、IgG、IgM 和 MHCII 呈阴性。聚合酶链反应分析显示存在 CD56、NKG2D、NKp30、NKp44、NKp46 和穿孔素,但不存在 CD16、Ly49 和颗粒酶 B mRNA。用 IL-2 处理 CNK-89 细胞不会改变激活受体的表达、TNFα 和 IFNγ 分泌和细胞毒活性,然而,单独使用 IL-12 或与 IL-15、IL-18 和 IL-21 联合处理会引起CNK-89 细胞系的颗粒酶 B 和 CD16 mRNA、IFNγ 分泌和细胞毒特性的增加。
更新日期:2021-03-27
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