Percomorpha is the major branch of Actinopteri, comprising most of the acanthopterygian fishes. The relationships among different Percomorpha groups remain uncertain because of high species diversity, thereby creating challenges for phylogenetic studies on teleosts. The phylogenetic problems of Percomorpha have not been solved using mitochondrial genes and a few nuclear genes. In this study, genomes from 34 representative species from the 7 major taxa of Percomorpha were selected to solve this problem. In all, 29 592 conserved amino acid sites from 206 single-copy orthologous genes were chosen for the reconstruction of phylogenetic trees. The results showed that Gobiaria, whose position is different from that in previous studies, was the earliest branching lineage. The phylogenetic groups in this study were consistent with previous results using mitochondrial DNA and nuclear genes. Thus, this study provides a new perspective and directions for further research on the Percomorpha branch and other higher-order meta-taxonomic relationships of fish. The genome data obtained may be essential for future investigations of phylogeny and molecular evolution.

Percomorpha是Actinopteri的主要分支，包括大多数棘翅类鱼类。由于高物种多样性，不同的Percomorpha组之间的关系仍然不确定，从而给硬骨鱼的系统发育研究带来了挑战。使用线粒体基因和一些核基因尚未解决过孔虫的系统发育问题。在这项研究中，选择了来自Percomorpha的7个主要分类群的34个代表性物种的基因组来解决此问题。从206个单拷贝直系同源基因中总共选择了29 592个保守氨基酸位点，以重建系统树。结果表明，戈比亚病菌的位置与以前的研究不同，是最早的分支谱系。本研究中的系统发育组与先前使用线粒体DNA和核基因的结果一致。因此，该研究为进一步研究鱼类的Percomorpha分支和其他高阶元分类学关系提供了新的视角和方向。获得的基因组数据对于系统发育和分子进化的未来研究可能是必不可少的。