Abstract

This study was aimed to investigate the effects of dietary fat sources and green tea extract (GTE) on gene expression associated with lipid metabolism and inflammation in broiler chicken. A total of 300 female Ross 308 broiler chicks were allocated to six dietary treatments in a completely randomised design with a factorial arrangement of two levels of GTE (0 and 500 mg/kg diet) × three levels of fat inclusion [without fat (control group), soybean oil (SO) and tallow (Ta)]. Liver fat (LF), liver methallothionin (LMT) content, and lipoprotein lipase (LPL), adipose triglyceride lipase (ATGL), tumour necrosis factor α (TNF-α), and interleukin (IL-6)] genes expression were investigated. Relative expression and statistical analyses of genes expression were assessed using REST software. The effect of added dietary fats was significant and improved performance parameters compared to the control group (p < .05). The highest abdominal fat and blood triglyceride, and the lowest carcase yield, were achieved in chicks fed SO-supplemented diet (p < .05). Chicks fed a diet supplemented with SO showed an overexpression and a decrease of LPL and ATGL compared to the Ta-supplemented and control groups, respectively (p < .05). GTE supplementation was effective on LPL and ATGL expression and ameliorated the effect of SO on abdominal fat percentage. TNF-α expression, LMT content, and relative weight of lymphoid organs were affected neither by GTE nor by the fat sources. There was no detection of IL-6 gene expression in adipose tissue. The results of this study demonstrated that diets rich in SO were capable increase fat deposition by an increase and decrease in LPL and ATGL gene expression, respectively. However inclusion of GTE in broiler diets alleviated the LPL-increasing and ATGL-reducing effects of SO and ameliorated the effect of SO on abdominal fat mass.

• Highlights

• Diets supplemented with SO increased abdominal fat mass by changes in LPL and ATGL gene expression.

• Obesity was not effective in the expression of inflammatory genes (TNF-α and IL-6), indicating a lack of connection between inflammation and obesity in broiler chicken.

• GTE (500 mg/kg diet) addition to the diets supplemented with SO ameliorated the effect of SO on LPL and ATGL gene expression and abdominal fat mass.

摘要

这项研究旨在调查饮食脂肪来源和绿茶提取物（GTE）对肉鸡脂质代谢和炎症相关基因表达的影响。总共300只雌性Ross 308肉鸡以完全随机的设计分配给六种饮食处理，将两种水平的GTE（0和500 mg / kg饮食）×三种脂肪含量[不含脂肪（对照组，对照组） ），大豆油（SO）和牛脂（Ta）]。肝脂肪（LF），肝金属硫蛋白（LMT）和脂蛋白脂肪酶（LPL），脂肪甘油三酸酯脂肪酶（ATGL），肿瘤坏死因子α（TNF-α）和白介素（IL-6））]研究了基因表达。使用REST软件评估相对表达和基因表达的统计分析。与对照组相比，添加膳食脂肪的效果显着且性能参数得到改善（p  <.05）。饲喂添加SO的雏鸡腹部脂肪和血液中的甘油三酸酯最高，,体产量最低（p  <.05）。与补充Ta的对照组和对照组相比，饲喂补充SO的雏鸡分别表现出过表达和LPL和ATGL降低（p  <.05）。GTE补充剂对LPL和ATGL有效表达并改善了SO对腹部脂肪百分比的影响。TNF-α表达，LMT含量和淋巴器官的相对重量均不受GTE和脂肪来源的影响。在脂肪组织中没有检测到IL-6基因表达。这项研究的结果表明，富含SO的饮食能够通过增加和减少LPL和ATGL基因表达来增加脂肪沉积。但是，在肉鸡日粮中添加GTE可以减轻SO对LPL的增加和ATGL的降低作用，并改善SO对腹部脂肪量的影响。

• 强调

• 补充SO的饮食通过LPL和ATGL基因表达的变化增加腹部脂肪量。

• 肥胖对于炎症基因（TNF-α和IL-6）的表达无效，表明肉鸡的炎症与肥胖之间缺乏联系。

• 在添加SO的饮食中添加GTE（500 mg / kg饮食）可改善SO对LPL和ATGL基因表达以及腹部脂肪量的影响。