Endocrine disrupting compounds (EDCs) are contaminants ubiquitously found in the environment, which pose a potential threat to aquatic and wetland ecosystems. Caiman latirostris, a crocodilian species that inhabits South American wetlands, is highly sensitive to EDC exposure. Previously, we reported that early postnatal exposure to EDCs such as Bisphenol A (BPA) and 17β-Estradiol (E₂) alters C. latirostris oviduct differentiation. The aim of this work was to elucidate the molecular mechanisms behind this alteration. To accomplish this, we established the ontogenic changes in histological features and the expression of Wnt-7a, Wnt-5a, β-catenin, FoxA2, desmin, and alpha smooth muscle actin (α-SMA) in the oviduct of C. latirostris. Then, we evaluated the effects of BPA and E₂ exposure on these histological features and protein expressions. Our results showed that during the postnatal differentiation of the oviduct the presence of histological features related to adenogenesis is associated with the levels of expression of FoxA2, β-catenin, Wnt-5a and Wnt-7a. Early postnatal exposure to BPA and E₂ decreased the presence of histological features related to adenogenesis and altered the levels of expression of FoxA2, β-catenin, Wnt-5a and Wnt-7a, as well as the desmin/α-SMA ratio. These findings suggest that altered levels of Wnt-7a, Wnt-5a, β-catenin and FoxA2 could play a role in the BPA and E₂–induced alteration in oviduct differentiation in C. latirostris. Thus, impaired adenogenesis and, probably, impaired reproduction in wildlife naturally exposed to BPA and other estrogenic agonists cannot be completely ruled out.

内分泌干​​扰化合物（EDC）是环境中普遍存在的污染物，对水生和湿地生态系统构成潜在威胁。Caiman latirostris是居住在南美湿地的鳄鱼物种，对EDC暴露高度敏感。以前，我们报道过产后早期暴露于双酚A（BPA）和17β-雌二醇（E ₂）等EDC会改变C. latirostris输卵管的分化。这项工作的目的是阐明这种改变背后的分子机制。为此，我们确定了C. latirostris输卵管中Wnt-7a，Wnt-5a，β-catenin，FoxA2，desmin和α平滑肌肌动蛋白（α-SMA）的组织学特征及其表达的本体变化。。然后，我们评估了BPA和E ₂暴露对这些组织学特征和蛋白质表达的影响。我们的结果表明，在输卵管的产后分化过程中，与腺发生有关的组织学特征的存在与FoxA2，β-catenin，Wnt-5a和Wnt-7a的表达水平有关。出生后早期暴露于BPA和E ₂减少了与腺发生有关的组织学特征的存在，并改变了FoxA2，β-catenin，Wnt-5a和Wnt-7a的表达水平以及结蛋白/α-SMA比率。这些发现表明，Wnt-7a，Wnt-5a，β-连环蛋白和FoxA2水平的改变可能在BPA和E ₂诱导的梭状芽胞杆菌输卵管分化中起作用。。因此，不能完全排除自然暴露于BPA和其他雌激素激动剂的野生动植物的腺生成受损和生殖受损的可能性。