Lipophilic extractives of wood cause pitch deposition during pulp and paper production, leading to serious economic losses and environmental pollution. Natural seasoning of wood material before pulping can reduce wood extractives, but there is no control of the pulp yield and quality. Pretreatment of the wood chips with selected fungi is regarded as better controlled seasoning with effective removal of problematic extractives. Commercially available fungal preparations are effective mostly for softwood, but not quite suitable for Acacia mangium and hybrid hardwood—the main pulpwood of Vietnam. In this paper, a fungal isolate TD36 was identified as white-rot basidiomycete Pleurotus sp. TD36 based on the biological characteristics and phylogenetic analysis. Having high activity of ligninolytic enzymes, amylase and protease, Pleurotus sp. TD36 was able to degrade lignin-like compounds (guaiacol, Remazol Brilliant Blue), starch and casein. Degradation of wood extractives was evaluated by laccase and sterol esterase assays of the fungus in shake flask cultures and in pretreatment of Acacia wood chips. Favorable conditions for wood pretreatment were selected, including: inoculum size, 1% v/w; wood moisture, 60% v/w; temperature, 28–30 °C and 30 days. Under such conditions, Pleurotus sp. TD36 removed about 68 ± 2.75% of wood acetone extractives soluble in chloroform. Of that, about 73–89% of fatty acids and fatty alcohols, 76% of free sterols and 63% of sterol ester, were removed. Fungal pretreatment did not significantly affect the cellulose content of wood and Kraft pulp yield.

木材的亲脂性提取物在纸浆和造纸过程中引起沥青沉积，从而导致严重的经济损失和环境污染。制浆前对木材进行自然调味可减少木材提取物，但无法控制纸浆的产量和质量。用精选的真菌对木片进行预处理被认为是可以有效去除有问题的提取物的较好的调味料。市售的真菌制剂主要对软木有效，但不适用于相思木和混合硬木（越南的主要纸浆木）。在本文中，真菌分离物TD36被鉴定为白腐担子菌白灵菇。sp。TD36根据生物学特性和系统发育分析。Pleurotus sp。具有较高的木质素分解酶，淀粉酶和蛋白酶的活性。TD36能够降解木质素样化合物（愈创木酚，Remazol亮蓝），淀粉和酪蛋白。通过摇瓶培养和相思木片预处理中真菌的漆酶和固醇酯酶分析评估了木材提取物的降解。选择合适的木材预处理条件，包括：接种物大小，1％v / w；木材水分，v / w的60％；温度，28–30°C和30天。在这种情况下，平菇sp。TD36去除了约68±2.75％的可溶于氯仿的丙酮丙酮提取物。其中，大约73-89％的脂肪酸​​和脂肪醇，76％的游离固醇和63％的固醇酯被去除。真菌预处理并未显着影响木材的纤维素含量和牛皮纸浆产量。