Nasopharyngeal myasis in horses with Rhinoestrus species has been associated with irritation of nasal cavities; pharynx and olfactory nerve injuries have varying degrees of clinical signs of symptoms until the death of animals. The aim of this study is to determine the cellular immune response against the infestation of Rhinoestrus spp. Therefore, the collection of Rhinoestrus spp. and its turbinates’ under aseptically condition. Morphological and molecular identification of the larvae to determine the larval species. Then; the turbinates’ subjected to RNA extraction against Tumor necrosis factor- alpha (TNF- α) through using of quantitative Real- time PCR. In the first larval instar (L1); the intensity of the larvae was recorded in relation with the genes expression as the animals had 1–10 larvae the TNF-α means was 5.55 ± 0.25. The animals that harbored 11–20 L1 the genes were 10.85 ± 0.14 in TNF- α. In the second and third larval instar (L2); the intensity of the larvae was recorded in the relation with the gene expression. In conclusion, the gene was expressed regularly against the infestation to Rhinoestrus usbekistanicus that provoked with the production of several immunological cells such as mast cell; eosinophils; macrophages and lymphocytes.

鼻发炎的马鼻咽部真菌病与鼻腔刺激有关。直到动物死亡，咽和嗅神经损伤都有不同程度的临床症状。这项研究的目的是确定针对犀牛种侵染的细胞免疫反应。因此，收集鼻发情spp。并在无菌条件下使其鼻甲化。幼虫的形态和分子鉴定，以确定幼虫的种类。然后; 鼻甲通过定量实时PCR对肿瘤坏死因子-α（TNF-α）进行RNA提取。在第一个幼虫龄期（L1）；记录幼虫的强度与基因表达的关系，因为动物有1–10个幼虫，TNF-α平均值为5.55±0.25。携带11–20 L1基因的动物的TNF-α为10.85±0.14。在第二和第三幼虫期（L2）；记录幼虫的强度与基因表达的关系。总之，该基因被定期表达，以抵抗美鼻犀引起几种免疫细胞如肥大细胞产生的物质；嗜酸性粒细胞 巨噬细胞和淋巴细胞。