Oral squamous cell carcinoma (OSCC) is the most common head and neck malignant tumor. Periodontitis, a common chronic inflammatory disease, has been proven to increase the risk of oral cancers. Porphyromonas gingivalis (P. gingivalis), the major pathogen in periodontal disease, was recently shown to promote the development of OSCC. However, the underlying mechanisms have not been defined. Emerging evidence suggests that P. gingivalis outer membrane vesicles (OMVs) contain different packaged small RNAs (sRNAs) with the potential to target host mRNA function and/or stability. In this study, we found that P. gingivalis OMVs promote the invasion and migration of OSCC cells in vitro. Further research showed that sRNA23392 was abundant in P. gingivalis OMVs and it promoted the invasion and migration of OSCC cells by targeting desmocollin-2 (DSC2). DSC2, a desmosomal cadherin family member, has been found to be involved in tumor progression. sRNA23392 inhibitors attenuated P. gingivalis OMV-induced migration and invasion of OSCC cells. Collectively, these findings are consistent with the hypothesis that sRNA23392 in P. gingivalis OMVs is a novel mechanism of the host–pathogen interaction, whereby P. gingivalis promotes the invasion and migration of OSCC.

中文翻译：

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牙龈卟啉单胞菌外膜囊泡包装的sRNA23392通过靶向desmocollin-2促进口腔鳞状细胞癌的迁移和侵袭

口腔鳞状细胞癌（OSCC）是最常见的头颈部恶性肿瘤。牙周炎是一种常见的慢性炎症性疾病，已被证明会增加患口腔癌的风险。牙龈卟啉单胞菌（P. gingivalis）是牙周病的主要病原体，最近被证明可以促进OSCC的发展。但是，尚未定义基本机制。新兴证据表明，牙龈卟啉单胞菌外膜囊泡（OMV）包含不同包装的小RNA（sRNA），具有靶向宿主mRNA功能和/或稳定性的潜力。在这项研究中，我们发现牙龈卟啉单胞菌OMV促进体外OSCC细胞的侵袭和迁移。进一步的研究表明，sRNA23392在牙龈卟啉单胞菌OMVs通过靶向desmocollin-2（DSC2）促进了OSCC细胞的侵袭和迁移。已发现桥粒钙黏着蛋白家族成员DSC2与肿瘤进展有关。sRNA23392抑制剂减弱了牙龈卟啉单胞菌OMV诱导的OSCC细胞迁移和侵袭。总的来说，这些发现与假牙单胞菌OMV中的sRNA23392是宿主-病原体相互作用的新机制的假说是一致的，由此牙龈假单胞菌促进了OSCC的侵袭和迁移。