Dose-response modeling of in vitro micronucleus test (IVMNT) data was evaluated to determine if the approach has value in discriminating among different tobacco products. Micronucleus responses were generated in L5178Y/Tk^+/− mouse lymphoma cells and TK6 human lymphoblastoid cells from a series of whole smoke solutions (WSSs) expected to have different levels of genotoxicity based on differences in their machine-generated smoke constituents. Eight WSSs were prepared by machine smoking different numbers (20 or 60) of two commercial cigarettes (Marlboro Silver or Red) under International Standardization Organization (ISO) or Health Canada Intense (HCI) smoking machine regimens and tested in the two cell lines with and without rat liver S9 activation. The S9-mediated IVMNT dose-response data from the WSSs were evaluated with PROAST software and Benchmark Doses (BMDs) and their upper and lower confidence intervals (CIs) were generated. IVMNT data differed based on the number and type of cigarettes smoked and smoking machine regimen. The IVMNT responses produced in mouse lymphoma cells generally were greater than in TK6 cells, but the ability of the two cell types to differentiate between WSSs was similar. The results indicate that BMD potency ranking was useful for differentiating between IVMNT responses.

对体外微核测试（IVMNT）数据的剂量反应模型进行了评估，以确定该方法是否具有区分不同烟草产品的价值。来自一系列全烟雾溶液 (WSS) 的 L5178Y/ Tk ^+/-小鼠淋巴瘤细胞和 TK6 人淋巴母细胞产生微核反应，预计根据机器产生的烟雾成分的差异，它们具有不同水平的遗传毒性。根据国际标准化组织 (ISO) 或加拿大卫生部强烈 (HCI) 吸烟机方案，通过机器吸烟不同数量（20 或 60）两种商业香烟（万宝路银或红）制备八个 WSS，并在两种细胞系中进行测试没有大鼠肝脏 S9 激活。使用 PROAST 软件和基准剂量 (BMD) 评估来自 WSS 的 S9 介导的 IVMNT 剂量反应数据，并生成其上限和下限置信区间 (CI)。 IVMNT 数据因吸烟数量和类型以及吸烟机养生方式而异。小鼠淋巴瘤细胞产生的 IVMNT 反应通常大于 TK6 细胞，但这两种细胞类型区分 WSS 的能力相似。结果表明 BMD 效力排名对于区分 IVMNT 反应很有用。